酶 - 抗体偶联物制备的快速微量方法。
Rapid micromethod for preparation of enzyme-antibody conjugates.
作者信息
Parkinson A J, Scott E N, Muchmore H G
出版信息
J Clin Microbiol. 1982 Apr;15(4):737-9. doi: 10.1128/jcm.15.4.737-739.1982.
Abstract
A rapid method is described for labeling antibody with alkaline phosphatase by one-step glutaraldehyde linkage. The method involves the centrifugation of a small volume of an enzyme and antibody mixture through a minicolumn packed with hydrated Sephadex. This procedure rapidly removes ammonium sulfate and glutaraldehyde from the enzyme-antibody mixture and results in the efficient recovery of conjugated antibody without significant dilution.
摘要
本文描述了一种通过一步戊二醛连接用碱性磷酸酶标记抗体的快速方法。该方法包括将少量酶和抗体混合物通过装有水合葡聚糖凝胶的微型柱进行离心。此过程可快速从酶-抗体混合物中去除硫酸铵和戊二醛，并能有效回收偶联抗体，且不会显著稀释。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.用戊二醛将酶与蛋白质偶联。偶联物用于抗原和抗体的检测。
Immunochemistry. 1969 Jan;6(1):43-52. doi: 10.1016/0019-2791(69)90177-3.
3
A continuous spectrophotometric method for measuring the activity of serum alkaline phosphatase.一种用于测定血清碱性磷酸酶活性的连续分光光度法。
Clin Chem. 1966 Feb;12(2):70-89.
4
A rapid method for desalting small volumes of solution.一种用于小体积溶液脱盐的快速方法。
Anal Biochem. 1973 Sep;55(1):328-30. doi: 10.1016/0003-2697(73)90325-4.
5
Protein-protein coupling reactions and the applications of protein conjugates.蛋白质-蛋白质偶联反应及蛋白质缀合物的应用。
Clin Chim Acta. 1976 Jul 1;70(1):1-31. doi: 10.1016/0009-8981(76)90002-4.
6
A simple centrifuge column for desalting protein solutions.一种用于蛋白质溶液脱盐的简易离心柱。
Anal Biochem. 1979 Nov 15;100(1):184-7. doi: 10.1016/0003-2697(79)90130-1.
7
A rapid method for the purification of antibody--enzyme conjugates.一种纯化抗体 - 酶偶联物的快速方法。
Can J Biochem. 1979 Mar;57(3):286-8. doi: 10.1139/o79-036.
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