Koike M, Komori A
J Oral Pathol. 1982 Apr;11(2):151-8. doi: 10.1111/j.1600-0714.1982.tb00151.x.
A new human epithelial cell line (MK-1) has been established from a well differentiated squamous cell carcinoma of the gingiva. It has been subcultured 90 times during the past 23 months. The population doubling time of the MK-1 cell line was 28 h. A colony forming ability with 18% plating efficiency was shown. Chromosomal analysis revealed an aneuploid human male karyotype with 22 marker chromosomes. The model chromosome number was 75. Scanning electron micrographs of MK-1 cells showed numerous microvilli and intercellular bridges. Transmission electron micrographs of MK-1 cells showed epithelial characteristics with desmosomes, tonofilaments and membrane-coating granules. Hetero-transplantation of cultured MK-1 cells produced tumors, the histology of which was similar to that of original ones. The ultrastructure of the tumors showed the presence of abundant tonofibrils and keratohyalin granules. This MK-1 cell line is a model useful for the study of keratinization of squamous cell carcinomas.
已从牙龈高分化鳞状细胞癌中建立了一种新的人上皮细胞系（MK-1）。在过去23个月中，该细胞系已传代培养90次。MK-1细胞系的群体倍增时间为28小时。其具有18%的接种效率的集落形成能力。染色体分析显示为非整倍体男性核型，有22条标记染色体。模式染色体数为75条。MK-1细胞的扫描电子显微镜照片显示有大量微绒毛和细胞间桥。MK-1细胞的透射电子显微镜照片显示具有桥粒、张力丝和膜包颗粒的上皮特征。培养的MK-1细胞异体移植产生肿瘤，其组织学与原肿瘤相似。肿瘤的超微结构显示存在丰富的张力原纤维和透明角质颗粒。该MK-1细胞系是研究鳞状细胞癌角质化的有用模型。
