Nishihira T, Kasai M, Mori S, Watanabe T, Kuriya Y, Suda M, Kitamura M, Hirayama K, Akaishi T, Sasaki T
Gan. 1979 Oct;70(5):575-84.
Two epithelial cell lines (TE-1 and TE-2) have been established from a well or poorly differentiated human squamous cell carcinoma of the esophagus. TE-1 has been subcultured 120 times during 2 years and 10 months, and TE-2, 50 times for almost 2 years. Cultured cells grew as isolated and piled-up colonies of epithelial cells. The average doubling time of the TE-1 cell line was 60 hr and that of TE-2, 72 hr. Distinctive marker chromosomes and a male karyotype were present in TE-1, but no marker chromosomes were seen in TE-2. Scanning electron microscopic examinations of both TE-1 and TE-2 confirmed the presence of desmosomes and interdigitated microvilli. Transmission electron micrographs of TE-1 showed the presence of abundant cell organelles, and a few organelles were found in the scanty cytoplasm of TE-2. There was a marked difference in the cell organelles between TE-1 and TE-2. Heterotransplantation of the cultured TE-1 and TE-2 cells produced tumors, the histological appearance of which was similar to that of the original ones. The carcinoembryonic antigen level of the medium in the confluent culture of TE-2 was 270 ng/10(6) cells. In the cytoplasm of TE-1 cells the number of paracrystals, which were produced by treatment with vinblastine sulfate, increased by the addition of cholera toxin to the medium.
已从人食管高分化或低分化鳞状细胞癌中建立了两种上皮细胞系（TE - 1和TE - 2）。TE - 1在2年10个月内传代培养了120次，TE - 2在近2年内传代培养了50次。培养的细胞以分离的上皮细胞堆积菌落的形式生长。TE - 1细胞系的平均倍增时间为60小时，TE - 2为72小时。TE - 1存在独特的标记染色体和男性核型，但TE - 2未见标记染色体。对TE - 1和TE - 2进行扫描电子显微镜检查，均证实存在桥粒和指状微绒毛。TE - 1的透射电子显微镜照片显示有丰富的细胞器，而TE - 2细胞质稀少，仅发现少量细胞器。TE - 1和TE - 2的细胞器存在明显差异。培养的TE - 1和TE - 2细胞异种移植产生肿瘤，其组织学外观与原肿瘤相似。TE - 2汇合培养物培养基中的癌胚抗原水平为270 ng/10(6)个细胞。在TE - 1细胞的细胞质中，用硫酸长春碱处理产生的副晶体数量，通过向培养基中添加霍乱毒素而增加。
