Kuriya Y, Kitamura M, Akaishi T, Hirayama K, Sekine Y, Nishihira T, Kasai M
Tohoku J Exp Med. 1983 Apr;139(4):377-87. doi: 10.1620/tjem.139.377.
A new epithelial cell line (TE-3) has been established from a metastatic lesion at the right chest wall which was originated from a well differentiated human squamous cell carcinoma of the esophagus. TE-3 has been subcultured more than 150 times for 3 years and 1 month. Cultured cells have grown as isolated colonies of epithelial cells. The average doubling time of the TE-3 cell line was 48 hr and the plating efficiency was 10 to 40% in MEM supplemented with 8% fetal calf serum. Distinctive marker chromosomes and a male karyotype were present in TE-3. Electron microscopic examination of the TE-3 cells disclosed the presence of desmosomes and microvilli in connection of the cells which were rich in cell organelles. In early passages of culture, the cytoplasm of cells was slightly positive with PAS stain but negative with Sudan III or mucicarmin stain. Heterotransplantation of the culture cells to BALB/c nude mice produced tumors, the histological appearance of which was similar to that of the original one. The carcinoembryonic antigen level of the medium in the confluent culture of TE-3 was 2 ng/10(4) cells.
一种新的上皮细胞系（TE - 3）已从右胸壁的转移性病变中建立，该病变起源于高分化的人食管鳞状细胞癌。TE - 3已传代培养超过150次，历时3年1个月。培养的细胞以孤立的上皮细胞集落形式生长。在补充有8%胎牛血清的MEM中，TE - 3细胞系的平均倍增时间为48小时，接种效率为10%至40%。TE - 3中存在独特的标记染色体和男性核型。对TE - 3细胞的电子显微镜检查发现，细胞间存在桥粒和微绒毛，且细胞富含细胞器。在培养的早期传代中，细胞的细胞质对PAS染色呈弱阳性，但对苏丹III或黏液卡红染色呈阴性。将培养细胞异种移植到BALB/c裸鼠体内产生了肿瘤，其组织学外观与原肿瘤相似。TE - 3汇合培养物培养基中的癌胚抗原水平为2 ng/10⁴细胞。
