Otsubo Y, Kameyama Y
J Oral Pathol. 1982 Apr;11(2):159-73. doi: 10.1111/j.1600-0714.1982.tb00152.x.
In this study, plaque-like lesions, epithelial hyperplasias, and papillary invasive carcinomas were produced in the tongues of hamsters by the application of DMBA combined with trauma for 16 weeks. The junction between the neoplastic epithelial cells and the connective tissue was studied ultrastructurally. In plaque-like lesions, the basal lamina was partially decreased in both thickness and density or partially discontinuous. In epithelial hyperplasias, cytoplasmic projections were observed in the portion of the basal cell near the intercellular space, and the basal lamina was absent around these cytoplasmic projections. In papillary invasive carcinomas, however, numerous cytoplasmic projections extended from various portions of the basal surface of the basal cells into the underlying connective tissue. The basal lamina was also absent around these projections. In epithelial hyperplasias and papillary invasive carcinomas, some of the cytoplasmic projections appeared almost empty, some contained a small number of tonofilaments and ribosomes, and others were filled with a large number of ribosomes but did not contain the tonofilaments. The collagen fibrils were markedly reduced in number in the area around cytoplasmic projections. The cytoplasm of some fibroblasts in that area contained many intracellular collagen fibrils.
在本研究中，通过应用二甲基苯并蒽（DMBA）并结合创伤处理16周，在仓鼠舌部产生了斑块状病变、上皮增生和乳头状浸润癌。对肿瘤上皮细胞与结缔组织之间的交界处进行了超微结构研究。在斑块状病变中，基膜的厚度和密度部分降低或部分不连续。在上皮增生中，在靠近细胞间隙的基底细胞部分观察到细胞质突起，并且在这些细胞质突起周围没有基膜。然而，在乳头状浸润癌中，许多细胞质突起从基底细胞基底表面的各个部分延伸到下方的结缔组织中。这些突起周围也没有基膜。在上皮增生和乳头状浸润癌中，一些细胞质突起几乎是空的，一些含有少量张力细丝和核糖体，而另一些则充满大量核糖体但不含张力细丝。在细胞质突起周围区域，胶原纤维数量明显减少。该区域一些成纤维细胞的细胞质中含有许多细胞内胶原纤维。
