囊性纤维化中循环免疫复合物的分离与鉴定
Isolation and characterization of circulating immune complexes in cystic fibrosis.
作者信息
Moss R B, Hsu Y P
出版信息
Clin Exp Immunol. 1982 Feb;47(2):301-8.
Abstract
A methodology for the isolation and immunologic characterization of IgG-containing circulating immune complexes (IgG-CIC) as detected by the 125IClq-binding assay (ClqBA) is described. We applied this methodology to sera from patients with cystic fibrosis (CF), both positive and negative for IgG-CIC. We used latex-fixation-positive rheumatoid arthritis sera and normal human sera as positive and negative controls, respectively. All IgG-CIC-positive serum samples from CF patients were found to contain antibodies against Pseudomonas aeruginosa in the isolated complexes. Some patients also had antibodies in CIC specific for Staphylococcus aureus and Candida albicans. CIC specificity corresponded to respiratory tract colonization for each patient.
摘要
本文描述了一种通过¹²⁵I-Clq结合试验（ClqBA）检测含IgG循环免疫复合物（IgG-CIC）并进行分离和免疫特性鉴定的方法。我们将此方法应用于囊性纤维化（CF）患者的血清，这些患者的IgG-CIC检测结果有阳性和阴性之分。我们分别使用乳胶固定阳性的类风湿性关节炎血清和正常人血清作为阳性和阴性对照。发现所有CF患者的IgG-CIC阳性血清样本在分离出的复合物中均含有抗铜绿假单胞菌的抗体。一些患者的CIC中还具有针对金黄色葡萄球菌和白色念珠菌的抗体。CIC的特异性与每位患者呼吸道的定植情况相符。
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