Lagacé J, Mercier J, Fréchette M, Fournier D, Dubreuil M, Lamarre A, Lapointe J R, Montplaisir S
Department of Microbiology and Immunology, Université de Montréal, Quebec, Canada.
J Clin Lab Immunol. 1989 Sep;30(1):7-11.
Serum samples from 37 patients with cystic fibrosis (CF), whose lungs were colonized by Pseudomonas aeruginosa, were tested in a 1 yr prospective study to examine a possible relationship between levels of circulating immune complexes (CIC) and the following parameters: level of specific antibodies to P. aeruginosa; relative importance of P. aeruginosa mucoid and non-mucoid strains isolated from sputum; the forced expiratory volume (FEV1; percentage predicted); the chest X-Ray score (Brasfield system) and the clinical score (Shwachman system). Reactivity of CIC against P. aeruginosa, Staphylococcus aureus, Haemophilus influenzae and Escherichia coli antigens were also assayed. We found that the FEV1, the chest X-Ray and the clinical scores were significantly lower in patients with high levels of CIC than in those with normal levels of CIC (p less than 0.001 for each). We also found that the level of IgG antibodies against P. aeruginosa was significantly higher (p less than 0.001) in patients with high levels of CIC than in those with normal levels of CIC. 78% of patients with high levels of CIC had predominantly mucoid P. aeruginosa isolates whereas only 21% of patients with normal levels of CIC had also predominantly mucoid P. aeruginosa isolates. Specific antibodies to P. aeruginosa were detected in all CIC isolated by polyethylene glycol precipitations from CF patients exhibiting both high levels of CIC and inferior pulmonary status. Our findings support the hypothesis that a high level of CIC in association with an aggressive humoral response to P. aeruginosa correlates with defective pulmonary status in cystic fibrosis.(ABSTRACT TRUNCATED AT 250 WORDS)
在一项为期1年的前瞻性研究中，对37例肺部被铜绿假单胞菌定植的囊性纤维化（CF）患者的血清样本进行了检测，以研究循环免疫复合物（CIC）水平与以下参数之间的可能关系：抗铜绿假单胞菌特异性抗体水平；从痰液中分离出的铜绿假单胞菌黏液型和非黏液型菌株的相对重要性；用力呼气量（FEV1；预测百分比）；胸部X线评分（布拉斯菲尔德系统）和临床评分（施瓦克曼系统）。还检测了CIC对铜绿假单胞菌、金黄色葡萄球菌、流感嗜血杆菌和大肠杆菌抗原的反应性。我们发现，CIC水平高的患者的FEV1、胸部X线和临床评分显著低于CIC水平正常的患者（每项p均小于0.001）。我们还发现，CIC水平高的患者抗铜绿假单胞菌IgG抗体水平显著高于CIC水平正常的患者（p小于0.001）。CIC水平高的患者中78%主要分离出黏液型铜绿假单胞菌，而CIC水平正常的患者中只有21%也主要分离出黏液型铜绿假单胞菌。在从CIC水平高且肺部状况较差的CF患者中通过聚乙二醇沉淀分离出的所有CIC中均检测到抗铜绿假单胞菌特异性抗体。我们的研究结果支持以下假设：CIC水平高与对铜绿假单胞菌的强烈体液反应相关，这与囊性纤维化患者肺部状况不佳有关。（摘要截短于250字）
