Cooper M D, Floyd S A
Infect Immun. 1982 Apr;36(1):363-70. doi: 10.1128/iai.36.1.363-370.1982.
Unstimulated resident peritoneal macrophages were harvested from complement-sufficient (C5+) and complement-deficient (C5-) mice by peritoneal lavage and cultured for 14 h. Adherence to cover slips was determined, and the monolayer was infected with transparent T1 gonococci. At various times after infection, the macrophages were observed for both attachment and phagocytosis of the gonococci by scanning and transmission electron microscopy. this analysis indicated that C5+ macrophages were capable of immediate phagocytosis of gonococci, with maximal phagocytosis occurring by 60 to 90 min. In contrast, C5- macrophages had a greater lag time before initiation of phagocytosis; this event was started by 30 min and completed by 90 min. The intracellular gonococci which were phagocytized by either C5+ or C5- mice were completely killed after 30 min of incubation. It appears that C5- mice are at a disadvantage in the early kinetics of the phagocytosis of gonococci, but that this does not affect the ultimate intracellular destruction of gonococci.
通过腹腔灌洗从补体充足（C5 +）和补体缺陷（C5 -）小鼠中收获未刺激的常驻腹膜巨噬细胞，并培养14小时。测定其对盖玻片的粘附性，然后用透明的T1淋球菌感染单层细胞。在感染后的不同时间，通过扫描电子显微镜和透射电子显微镜观察巨噬细胞对淋球菌的附着和吞噬情况。该分析表明，C5 +巨噬细胞能够立即吞噬淋球菌，在60至90分钟时吞噬作用达到最大。相比之下，C5 -巨噬细胞在开始吞噬之前有更长的延迟时间；吞噬作用在30分钟时开始，90分钟时完成。被C5 +或C5 -小鼠吞噬的细胞内淋球菌在孵育30分钟后被完全杀死。似乎C5 -小鼠在淋球菌吞噬作用的早期动力学方面处于劣势，但这并不影响淋球菌最终在细胞内的破坏。
