小鼠腹腔巨噬细胞对淋病奈瑟菌吞噬作用的定量测定。
Quantitative measurement of phagocytosis of Neisseria gonorrhoeae by mouse peritoneal macrophages.
作者信息
Jones R B, Buchanan T M
出版信息
Infect Immun. 1978 Jun;20(3):732-8. doi: 10.1128/iai.20.3.732-738.1978.
Abstract
The simultaneous labeling of gonococci with [6-3H]uracil and of mouse peritoneal macrophages with L-[U-14C]leucine permits a quantitative assessment of the association of gonococci with macrophages under various experimental conditions. Colony-type T1 (piliated) gonococci associated more than T4 (nonpiliated) organisms at 4 degrees C, but at 37 degrees C the association of T4 gonococci with macrophages exceeded that for the T1 organisms. The association of T1 gonococci with macrophages could be enhanced as much as 70-fold by homologous rabbit antisera prepared against whole, formaldehyde-treated organisms. This immune enhancement represented primarily increased phagocytosis rather than surface attachment, as shown by its inhibition at 4 degrees C or with 2-deoxyglucose. The data further suggested that this enhanced phagocytosis was mediated via the Fc receptor for immunoglobulin G.
摘要
用[6-³H]尿嘧啶对淋球菌进行标记，并用L-[U-¹⁴C]亮氨酸对小鼠腹腔巨噬细胞进行标记，这样就能在各种实验条件下对淋球菌与巨噬细胞的结合进行定量评估。在4℃时，菌落类型为T1（有菌毛）的淋球菌比T4（无菌毛）菌株与巨噬细胞的结合更多，但在37℃时，T4淋球菌与巨噬细胞的结合超过了T1菌株。用针对经甲醛处理的完整菌体制备的同源兔抗血清，可使T1淋球菌与巨噬细胞的结合增强达70倍之多。如在4℃或用2-脱氧葡萄糖处理时所显示的那样，这种免疫增强主要表现为吞噬作用的增加，而非表面附着。数据进一步表明，这种增强的吞噬作用是通过免疫球蛋白G的Fc受体介导的。
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