Schillaci M, Martin S E, Milner J A
Mutat Res. 1982 Mar;101(1):31-7. doi: 10.1016/0165-1218(82)90162-8.
The influence of dietary selenium on the mutagenic activation of 7,12-dimethylbenz[a]anthracene (DMBA) by rat liver S9 was studied using the Ames test. Rats received supplemental selenium, as sodium selenite, in the drinking water or in the diet. All rats additionally received 0, 20, 50, 100, or 500 mg Aroclor 1254 per kg body weight. Revertant counts decreased 72 and 31% at the 20- and 100-mg/kg induction levels, respectively, with S9 preparations from rats given selenium supplementation, compared to controls. No significant effects of selenium on S9 preparations was observed in rats treated with 500 mg/kg Aroclor. Preparations of S9 from rats receiving 2.5 ppm Se in their diet produced 46, 84 and 70% less revertants than controls at the 20-, 50- and 100-mg/kg induction levels. Increasing the selenium concentration in the diet to 5 ppm reduced the revertant counts by 71, 68 and 65%, at the 20-, 50- and 100-mg/kg induction level of Aroclor, respectively. Dietary selenium supplementation was shown to decrease the mutagenic activation of DMBA by liver microsomes. These studies indicate that in vivo selenium supplementation may reduce susceptibility to the action of various carcinogens.
采用艾姆斯试验研究了膳食硒对大鼠肝脏S9激活7,12 - 二甲基苯并[a]蒽（DMBA）致突变作用的影响。大鼠通过饮用水或饮食摄入补充硒，即亚硒酸钠。所有大鼠还额外接受每千克体重0、20、50、100或500毫克的多氯联苯混合物Aroclor 1254。与对照组相比，在20毫克/千克和100毫克/千克诱导水平下，给予补充硒的大鼠的S9制剂的回复突变菌落数分别减少了72%和31%。在接受500毫克/千克Aroclor处理的大鼠中，未观察到硒对S9制剂有显著影响。在饮食中摄入2.5 ppm硒的大鼠的S9制剂，在20毫克/千克、50毫克/千克和100毫克/千克诱导水平下，产生的回复突变菌落数比对照组分别少46%、84%和70%。将饮食中的硒浓度提高到5 ppm，在Aroclor的20毫克/千克、50毫克/千克和100毫克/千克诱导水平下，回复突变菌落数分别减少了71%、68%和65%。膳食补充硒可降低肝脏微粒体对DMBA的致突变激活作用。这些研究表明，体内补充硒可能降低对各种致癌物作用的易感性。
