Fujisaki K, Takamatsu H, Kitaoka S, Suzuki K, Kamio T
Natl Inst Anim Health Q (Tokyo). 1981 Summer;21(2):73-9.
Direct immunofluorescence was first used successfully for the staining of Leucocytozoon caulleryi of different developmental stages, such as the 2nd generation schizont and merozoite, gametocyte, zygote, ookinete, and sporozoite. Fluorescent antibody of IgG nature prepared from sera of chickens over a period from the 21st to the 100th day after sporozoite inoculation was used in this experiment. The cross reactivity on different developmental stages of L. caulleryi in the direct fluorescent antibody test suggested that antigens common to different developmental stages, or shared antigens, might exist, as well as specific antigens so far well-known. The fluorescent intensity of oocyst sporozoite, and schizont contents was apparently lower than that of any other stage. This evidence might be elucidated by the antigenic incompletion.
直接免疫荧光法首次成功用于对卡氏住白细胞虫不同发育阶段进行染色，如第二代裂殖体和裂殖子、配子体、合子、动合子和子孢子。本实验使用了在接种子孢子后第21天至第100天期间从鸡血清中制备的IgG性质的荧光抗体。在直接荧光抗体试验中，卡氏住白细胞虫不同发育阶段的交叉反应表明，可能存在不同发育阶段共有的抗原，即共享抗原，以及迄今已知的特异性抗原。卵囊子孢子和裂殖体内容物的荧光强度明显低于其他任何阶段。这一证据可能是由于抗原不完整所致。
