利用结核分枝杆菌中的吡嗪酰胺酶活性作为快速测定吡嗪酰胺敏感性的方法。
Use of pyrazinamidase activity on Mycobacterium tuberculosis as a rapid method for determination of pyrazinamide susceptibility.
作者信息
McClatchy J K, Tsang A Y, Cernich M S
出版信息
Antimicrob Agents Chemother. 1981 Oct;20(4):556-7. doi: 10.1128/AAC.20.4.556.
Abstract
Pyrazinamidase activity in clinical isolates of Mycobacterium tuberculosis has been previously found to correlate with susceptibility to the antituberculosis drug pyrazinamide. The Wayne method for determining pyrazinamidase activity, a technique also utilized as an aid in identification of mycobacteria, and thin-layer chromatography method were found to be useful screening methods for susceptibility testing, since resistant strains are pyrazinamidase negative. These simple methods overcome the difficulty in growing M. tuberculosis at pH 5.5, as is required in the conventional method of susceptibility testing.
摘要
先前已发现结核分枝杆菌临床分离株中的吡嗪酰胺酶活性与对抗结核药物吡嗪酰胺的敏感性相关。用于测定吡嗪酰胺酶活性的韦恩方法（一种也用于辅助鉴定分枝杆菌的技术）和薄层色谱法被发现是有用的药敏试验筛选方法，因为耐药菌株为吡嗪酰胺酶阴性。这些简单方法克服了在常规药敏试验方法所要求的pH 5.5条件下培养结核分枝杆菌的困难。
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本文引用的文献
1
Activation of pyrazinamide and nicotinamide in acidic environments in vitro.吡嗪酰胺和烟酰胺在体外酸性环境中的活化作用。
Am Rev Tuberc. 1954 Oct;70(4):748-54. doi: 10.1164/art.1954.70.4.748.
2
Determination of drug susceptibility of mycobacteria to pyrazinamide in 7H10 agar.
Am Rev Respir Dis. 1967 Nov;96(5):1072-5. doi: 10.1164/arrd.1967.96.5.1072.
3
Pyrazinamide susceptibility and amidase activity of tubercle bacilli.结核杆菌的吡嗪酰胺敏感性及酰胺酶活性
Am Rev Respir Dis. 1967 Mar;95(3):461-9. doi: 10.1164/arrd.1967.95.3.461.
4
Simple pyrazinamidase and urease tests for routine identification of mycobacteria.用于分枝杆菌常规鉴定的简易吡嗪酰胺酶和脲酶试验
Am Rev Respir Dis. 1974 Jan;109(1):147-51. doi: 10.1164/arrd.1974.109.1.147.
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