van Reenen P C, Lötter M G, Heyns A D, de Kock F, Herbst C, Kotzé H, Pieters H, Minnaar P C, Badenhorst P N
Eur J Nucl Med. 1982;7(2):80-4. doi: 10.1007/BF00251649.
A single and practical approach to the in vivo quantification of 111indium-oxine labelled blood platelets with a scintillation camera and computer assisted imaging system was evaluated. Radioactivity of the 172 and 247 keV energies was measured in a phantom at various source distances from the collimator and the accuracy of anterior and posterior mode measurements compared with that of the geometrical mean (GM) method, with and without correction for Compton scatter (CS). Organ radioactivity, expressed as a percentage of whole body radioactivity, was determined in vivo in five baboons and the accuracy of the methods verified by post mortem quantification in the animals. Measurements in the anterior mode significantly overestimates hepatic and underestimates splenic radioactivity; posterior mode quantification reverses these results. Correction with the GM method made the accuracy and reproducibility very acceptable. Further correction for anterior-posterior attenuation and/or CS did not improve results materially. The GM method could readily be applied in five human subjects. This study showed that the GM method is an accurate and practical method for the in vivo quantification of organ and regional distribution of 111In-labelled platelets.
评估了一种使用闪烁相机和计算机辅助成像系统对111铟-氧肟酸标记的血小板进行体内定量的单一实用方法。在模体中，于距准直器不同源距离处测量了172和247 keV能量的放射性，并将前后模式测量的准确性与几何平均（GM）法进行比较，比较了有无康普顿散射（CS）校正的情况。以五只狒狒体内全身放射性的百分比表示的器官放射性，通过动物死后定量来验证这些方法的准确性。前位模式测量显著高估了肝脏放射性而低估了脾脏放射性；后位模式定量则使这些结果相反。用GM法校正后，准确性和可重复性非常令人满意。进一步校正前后衰减和/或CS并未实质性改善结果。GM法可轻松应用于五名人类受试者。本研究表明，GM法是一种准确且实用的方法，可用于体内定量111In标记血小板的器官和区域分布。
