Nguyen B Y, Peterson P K, Verbrugh H A, Quie P G, Hoidal J R
Infect Immun. 1982 May;36(2):504-9. doi: 10.1128/iai.36.2.504-509.1982.
Phagocytosis and killing by alveolar macrophages from humans, rabbits, rats, and hamsters, were compared in vitro. In the absence of serum opsonins, human alveolar macrophages could phagocytize Staphylococcus aureus Cowan I (protein A positive), but not S. aureus EMS (protein A negative) or Pseudomonas aeruginosa MN. In contrast, rabbit, rat, and hamster alveolar macrophages did not phagocytize S. aureus Cowan I or other nonopsonized bacteria. Human alveolar macrophages, but not other species, stained positively with fluorescein isothiocyanate-conjugated protein A. When opsonized bacterial were studied, phagocytosis by human, rabbit, and hamster alveolar macrophages was found to be mediated by both Fc and C3 receptors. However, only Fc receptor-mediated phagocytosis of bacteria was demonstrated for rat alveolar macrophages. Differences were also found in the kinetics of bacterial killing by alveolar macrophages from different species. Human and rabbit alveolar macrophages rapidly killed opsonized S. aureus Cowan I. However, bacterial killing by hamster alveolar macrophages proceeded at a slower rate, and rat alveolar macrophages completely failed to kill S. aureus. These significant differences in the function of alveolar macrophages from four different species emphasize the need to document the appropriateness of animal models before using them to predict the biological activities of human alveolar macrophages.
在体外比较了来自人类、兔子、大鼠和仓鼠的肺泡巨噬细胞的吞噬作用和杀菌作用。在没有血清调理素的情况下，人类肺泡巨噬细胞能够吞噬金黄色葡萄球菌考恩I型（蛋白A阳性），但不能吞噬金黄色葡萄球菌EMS型（蛋白A阴性）或铜绿假单胞菌MN型。相比之下，兔子、大鼠和仓鼠的肺泡巨噬细胞不能吞噬金黄色葡萄球菌考恩I型或其他未调理的细菌。人类肺泡巨噬细胞而非其他物种的肺泡巨噬细胞，用异硫氰酸荧光素偶联的蛋白A染色呈阳性。当研究调理后的细菌时，发现人类、兔子和仓鼠的肺泡巨噬细胞的吞噬作用由Fc和C3受体介导。然而，仅证明大鼠肺泡巨噬细胞对细菌的吞噬作用是由Fc受体介导的。在不同物种的肺泡巨噬细胞杀菌动力学方面也发现了差异。人类和兔子的肺泡巨噬细胞能迅速杀死调理后的金黄色葡萄球菌考恩I型。然而，仓鼠肺泡巨噬细胞的杀菌速度较慢，而大鼠肺泡巨噬细胞完全无法杀死金黄色葡萄球菌。来自四种不同物种的肺泡巨噬细胞功能的这些显著差异强调了在使用动物模型预测人类肺泡巨噬细胞的生物学活性之前，需要证明其适用性。
