枯草芽孢杆菌完整细胞、原生质体及不同rec突变体在质粒转化和转导中标记拯救现象的研究。
Study of the phenomenon of marker rescue in plasmid transformation and transduction of intact cells, protoplasts and different rec mutants of Bacillus subtilis.
作者信息
Prozorov A A, Bashkirov V I, Lakomova N M, Surikov N N
出版信息
Mol Gen Genet. 1982;185(2):363-4. doi: 10.1007/BF00330813.
PMID:6806568
Abstract
Marker rescue in plasmid transformation of competent cells of different rec mutants of B. subtilis was studied. In most cases the value of marker rescue decreased proportionally to reduction of plasmid transformation efficiency (although there were certain exceptions). Marker rescue was not observed either in plasmid transformation of protoplasts or in plasmid transduction of intact cells.
摘要
研究了枯草芽孢杆菌不同rec突变体感受态细胞在质粒转化中的标记拯救情况。在大多数情况下，标记拯救的值与质粒转化效率的降低成比例下降（尽管有某些例外）。在原生质体的质粒转化或完整细胞的质粒转导中均未观察到标记拯救现象。
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