Benzi G, Arrigoni E, Agnoli A, Raimondo S, Fulle D, Pastoris O, Curti D, Villa R F
Exp Gerontol. 1982;17(1):19-31. doi: 10.1016/0531-5565(82)90005-5.
The synaptosomal fraction obtained from the motor area of the cerebral cortex of normocapnic, normoxic or hypoxic "young adult," "mature" and "senescent" beagle dogs is incubated and analyzed for : ATP, ADP, AMP, creatine phosphate, pyruvate and lactate. The data are compared with those obtained from the whole controlateral cortical motor area, by the surface freezing technique. After hypoxic hypoxia /15 min; PaO2 = 17-19 mm Hg), the metabolite contents and ratios are differently affected by ageing when the evaluations are performed in the incubated synaptosomal preparation or in the controlateral whole cerebral tissue. In fact, ageing does not affect so much the cerebral changes that occur in the overall energetic state during the hypoxic assault in vivo, but rather those that the synaptosomes remember the tend to reverse during the subsequent incubation in vitro. The protective action of several drugs on the synaptosomal phosphorylation state is tested. Phenobarbital shows a quite broad, age-independent spectrum of action. (-)Eburnamonine and dihydroergocristine exhibits a more limited, age-dependent effectiveness, but are devoid of anesthetic action. Papaverine proves unable to affect the tested biochemical parameters.
从正常碳酸血症、常氧或低氧状态的“年轻成年”、“成熟”和“衰老”比格犬大脑皮质运动区获得的突触体部分进行孵育，并分析其中的三磷酸腺苷（ATP）、二磷酸腺苷（ADP）、一磷酸腺苷（AMP）、磷酸肌酸、丙酮酸和乳酸。通过表面冷冻技术，将这些数据与从对侧整个皮质运动区获得的数据进行比较。在低氧性缺氧（15分钟；动脉血氧分压=17 - 19毫米汞柱）后，当在孵育的突触体制剂或对侧全脑组织中进行评估时，代谢物含量和比率受衰老的影响不同。事实上，衰老对体内低氧攻击期间整体能量状态发生的大脑变化影响不大，而是对突触体在随后体外孵育期间倾向于逆转的那些变化影响较大。测试了几种药物对突触体磷酸化状态的保护作用。苯巴比妥显示出相当广泛的、与年龄无关的作用谱。（-）育亨宾和双氢麦角汀表现出更有限的、与年龄相关的有效性，但没有麻醉作用。罂粟碱被证明无法影响所测试的生化参数。
