Benzi G, Arrigoni E, Pastoris O, Raimondo S, Fulle D, Curti D, Villa R F
Eur Neurol. 1981;20(3):235-44. doi: 10.1159/000115241.
Synaptosomal preparations from the motor area of the cerebral cortex of normocapnic, normoxic or hypoxic untreated beagle dogs and phenobarbital-, papaverine-, and (-) eburnamonine-treated dogs were incubated for 10 min at 24 degrees C and analyzed for ATP, ADP, AMP creatine phosphate, pyruvate, and lactate. The data were compared with those obtained from the whole controlateral cortical motor area, by the surface-freezing technique. Both during normoxia and after hypoxic hypoxia (15 min, at PaO2 equal to 17-19 mm Hg) the metabolite contents and ratios were very different in the incubated synaptosomal preparations and in the whole cerebral tissues. As concerns the drug treatment, papaverine was always inactive, while (1) eburnamonine increased the synaptosomal phosphorylation state in hypoxic dogs, being ineffective on the glycolytic metabolites evaluated. Phenobarbital increased the synaptosomal phosphorylation state both in normoxic and in hypoxic animals, and was effective also on the glycolytic metabolites studied.
对正常碳酸血症、常氧或未经处理的低氧比格犬以及经苯巴比妥、罂粟碱和（-）埃布纳明处理的犬的大脑皮质运动区的突触体标本，在24℃孵育10分钟，然后分析其中的三磷酸腺苷（ATP）、二磷酸腺苷（ADP）、一磷酸腺苷（AMP）、磷酸肌酸、丙酮酸和乳酸。通过表面冷冻技术，将这些数据与从整个对侧皮质运动区获得的数据进行比较。在常氧期间以及低氧性低氧（15分钟，动脉血氧分压等于17 - 19毫米汞柱）后，孵育的突触体标本和整个脑组织中的代谢物含量及比率差异很大。关于药物治疗，罂粟碱始终无活性，而（-）埃布纳明增加了低氧犬突触体的磷酸化状态，对所评估的糖酵解代谢物无效。苯巴比妥在常氧和低氧动物中均增加了突触体的磷酸化状态，并且对所研究的糖酵解代谢物也有作用。
