A major cytoplasmic glucose-regulated protein is associated with the Rous sarcoma virus pp60src protein.

Previous studies have established that glucose deprivation of murine cells suppresses the synthesis of an Mr = 85,000 polypeptide. A protein of approximately the same molecular weight has been found to be associated with the transforming protein of Rous sarcoma virus, pp60src. The present study compares the Mr = 85,000 glucose-regulated protein with the pp60src-associated protein. By the criteria of mobility in sodium dodecyl sulfate-polyacrylamide gels and one-dimensional partial proteolytic peptide mapping the two proteins appear to be identical. It has previously been shown that the pp60src-associated protein is also identical with one of several proteins whose synthesis is induced after growth of cells at elevated temperatures or in the presence of arsenite and canavanine. Considering that it is involved in a number of complex response patterns, the name syndromin is proposed for this protein. These findings open the possibility of a fundamental interrelationship among the heat shock effect, regulation of protein synthesis by glucose deprivation, and oncogenic transformation by Rous sarcoma virus.