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测定玉米核酮糖-1,5-二磷酸羧化酶大亚基的翻译起始位点。

Determination of the translation start site of the large subunit of ribulose-1,5-bisphosphate carboxylase from maize.

机构信息

Roche Institute of Molecular Biology, Roche Research Center, 07110, Nutley, NJ, U.S.A..

出版信息

Plant Mol Biol. 1984 Nov;3(6):403-6. doi: 10.1007/BF00033388.

DOI:10.1007/BF00033388
PMID:24310574
Abstract

Sequence studies of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase from maize indicate the presence of two translation start sites, 18 bp apart. Each site is preceded by a suitable ribosome binding region. By using a simplified E. coli-based in vitro system which measures fromation of the first dipeptide of the gene product, we have determined that only the second methionine initiates translation.

摘要

玉米核酮糖-1,5-二磷酸羧化酶大亚基基因的序列研究表明，存在两个相距 18 个碱基的翻译起始位点。每个起始位点都有一个合适的核糖体结合区。我们使用了一个简化的基于大肠杆菌的体外系统来测量基因产物的第一个二肽的形成，结果表明只有第二个甲硫氨酸起始翻译。

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Plant Mol Biol. 1984 Nov;3(6):403-6. doi: 10.1007/BF00033388.
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本文引用的文献

1
Synthesis of the large subunit of ribulose-1,5-bisphosphate carboxylase in anin vitro partially definedE. coli system.在体外部分定义的大肠杆菌系统中合成核酮糖-1,5-二磷酸羧化酶大亚基。
Plant Mol Biol. 1983 Sep;2(5):279-90. doi: 10.1007/BF01578646.
2
Photochrome-mediated regulation of messenger RNAs for the small subunit of ribulose 1, 5-bisphosphate carboxylase and the light-harvesting chlorophyll a/b-protein in Lemna gibba.光致变色调节莱茵衣藻核酮糖 1,5-二磷酸羧化酶小亚基和捕光叶绿素 a/b 蛋白的信使 RNA。
Plant Mol Biol. 1981 Mar;1(1):35-51. doi: 10.1007/BF00023012.
3
Molecular cloning and sequence analysis of the cyanobacterial gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase.
藻蓝细菌核酮糖-1,5-二磷酸羧化酶/加氧酶大亚基基因的分子克隆和序列分析。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4050-4. doi: 10.1073/pnas.80.13.4050.
4
Isolation and sequence of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase from the cyanobacterium Anabaena 7120.从蓝藻鱼腥藻 7120 中分离并测定核酮糖-1,5-二磷酸羧化酶大亚基的基因序列。
Proc Natl Acad Sci U S A. 1983 Apr;80(7):1835-9. doi: 10.1073/pnas.80.7.1835.
5
Cloning, mapping, and in vitro transcription-translation of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase from spinach chloroplasts.菠菜叶绿体 RuBP 羧化酶大亚基基因的克隆、定位和体外转录-翻译。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3459-63. doi: 10.1073/pnas.78.6.3459.
6
Maize chloroplast DNA fragment encoding the large subunit of ribulosebisphosphate carboxylase.编码核酮糖二磷酸羧化酶大亚基的玉米叶绿体DNA片段。
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5487-91. doi: 10.1073/pnas.74.12.5487.
7
The nucleotide sequence of the tobacco chloroplast gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase.烟草叶绿体中核酮糖-1,5-二磷酸羧化酶/加氧酶大亚基基因的核苷酸序列。
Gene. 1982 Nov;20(1):91-102. doi: 10.1016/0378-1119(82)90090-7.
8
Translational control of ribosomal protein L10 synthesis occurs prior to formation of first peptide bond.核糖体蛋白L10合成的翻译控制发生在第一个肽键形成之前。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4261-4. doi: 10.1073/pnas.78.7.4261.
9
The gene for the large subunit of ribulose-1,5-bisphosphate carboxylase in Euglena gracilis chloroplast DNA: location, polarity, cloning, and evidence for an intervening sequence.纤细裸藻叶绿体DNA中1,5 - 二磷酸核酮糖羧化酶大亚基的基因：定位、极性、克隆及存在间隔序列的证据
Nucleic Acids Res. 1982 Jun 11;10(11):3427-44. doi: 10.1093/nar/10.11.3427.
10
Use of different tRNASer isoacceptor species in vitro to discriminate between the expression of plasmid genes.在体外使用不同的丝氨酸tRNA同工受体种类来区分质粒基因的表达。
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1466-8. doi: 10.1073/pnas.79.5.1466.