Botany School, University of Cambridge, Cambridge CB2 3EA, UK.
EMBO J. 1985 Jun;4(6):1381-8. doi: 10.1002/j.1460-2075.1985.tb03790.x.
The gene for CF(0) subunit I of ATP synthase has been located in wheat chloroplast DNA, between the genes for CF(0) subunit III and alpha subunit of CF(1). Nucleotide sequencing and analysis of RNA-DNA hybrids indicated that the gene is interrupted by an 823-bp intron which has boundaries similar to those previously described for the introns in protein-coding chloroplast genes of Euglena gracilis. The deduced amino acid sequence of CF(0) subunit I indicates a polypeptide of 183 amino acid residues. However, N-terminal amino acid sequencing of the mature spinach CF(0) subunit I suggests that the protein is synthesised with a N-terminal extension of 17 amino acid residues and is processed to give a protein of mol. wt. 19 001 of 166 amino acids residues. The mature CF(0) subunit I shows similarities in primary and predicted secondary structure to F(0) subunit b of Escherichia coli ATP synthase. A major transcript of 3.3 kb containing sequences from the genes for CF(0) subunit III, subunit I and CF(1) subunit alpha has been observed by RNA-DNA hybridisation.
CF（0）亚基 I 的基因已被定位在小麦叶绿体 DNA 中，位于 CF（0）亚基 III 和 CF（1）的α亚基之间。核苷酸测序和 RNA-DNA 杂交分析表明，该基因被一个 823bp 的内含子所打断，该内含子的边界与先前在 Euglena gracilis 的叶绿体蛋白编码基因中的内含子相似。CF（0）亚基 I 的推断氨基酸序列表明其是由 183 个氨基酸残基组成的多肽。然而，成熟菠菜 CF（0）亚基 I 的 N-末端氨基酸测序表明，该蛋白的 N-末端延伸了 17 个氨基酸残基，经过加工后得到分子量为 19001 的 166 个氨基酸残基的成熟蛋白。成熟的 CF（0）亚基 I 在一级和预测的二级结构上与大肠杆菌 ATP 合酶的 F（0）亚基 b 相似。通过 RNA-DNA 杂交观察到一个含有 CF（0）亚基 III、I 和 CF（1）亚基α基因序列的 3.3kb 主要转录物。
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