Lacy P E, Finke E H, Janney C G, Davie J M
Transplantation. 1982 Jun;33(6):588-92. doi: 10.1097/00007890-198206000-00004.
Individual rat islets could be aggregated into single megaislets in vitro and the megaislets remained morphologically and functionally intact after a 7-day period of culture in the presence of 95% O2 and 5% CO2. Cultured rat megaislets transplanted beneath the renal capsule of diabetic mice produced normoglycemia in the recipients and the survival of the xenografts was markedly prolonged by the 7-day exposure of a high oxygen tension. A single injection of antilymphocyte sera to mouse and rat lymphocytes into the recipients receiving cultured megaislets did not produce a further increase in the percentage of survival of the grafts at 70 days after transplantation. Lymphoid aggregates were present around xenografts of cultured negaislets at 60 and 90 days after transplantation. This lymphoid reaction did not interfere with the function of the xenografts since the recipients were normoglycemic and removal of the grafts resulted in a rapid return to a diabetic state. Intraportal and intrasplenic transplants of cultured rat megaislets did not survive as long as the xenografts of megaislets transplanted beneath the renal capsule. The renal subcapsule site apparently provided some immunological advantage for delaying acute rejection since transplants of individual, fresh rat islets survived for twice as long under the renal capsule as compared wtih intraportal transplants of fresh rat islets.
单个大鼠胰岛在体外可聚集成单个大胰岛，在含95% O₂和5% CO₂的条件下培养7天后，大胰岛在形态和功能上仍保持完整。将培养的大鼠大胰岛移植到糖尿病小鼠的肾被膜下，可使受体血糖正常，高氧张力暴露7天可显著延长异种移植物的存活时间。在接受培养的大胰岛的受体中，单次注射抗淋巴细胞血清至小鼠和大鼠淋巴细胞，在移植后70天时并未使移植物存活百分比进一步增加。移植后60天和90天时，培养的大胰岛异种移植物周围出现淋巴样聚集物。这种淋巴样反应并未干扰异种移植物的功能，因为受体血糖正常，移除移植物会导致迅速恢复到糖尿病状态。培养的大鼠大胰岛门静脉内移植和脾内移植的存活时间不如移植到肾被膜下的大胰岛异种移植物长。肾被膜下部位显然为延迟急性排斥反应提供了一些免疫优势，因为单个新鲜大鼠胰岛在肾被膜下的存活时间是新鲜大鼠胰岛门静脉内移植存活时间的两倍。
