来自大肠杆菌的微菌素15m：抗生素作用机制
Microcin 15m from Escherichia coli: mechanism of antibiotic action.
作者信息
Aguilar A, Pérez-Díaz J C, Baquero F, Asensio C
出版信息
Antimicrob Agents Chemother. 1982 Mar;21(3):381-6. doi: 10.1128/AAC.21.3.381.
Abstract
It has been previously established that a high proportion of enterobacteria isolated from the intestinal content of humans, mostly Escherichia coli strains, secrete into the culture media antibiotic substances of low molecular weight, which have been called microcins. It was also found that the synthesis of these antibiotics is determined by plasmids. In this paper, a method for the purification of microcin 15m is described, and experimental data are given on the mechanism of its action. The data indicate that this action is based mainly on the inhibition of the first enzyme of the methionine biosynthetic pathway, homoserine-O-transsuccinylase, a similar way to the allosteric inhibition caused by this amino acid.
摘要
先前已经确定，从人类肠道内容物中分离出的大部分肠杆菌（主要是大肠杆菌菌株）会向培养基中分泌低分子量的抗生素物质，这些物质被称为微菌素。还发现这些抗生素的合成由质粒决定。本文描述了一种纯化微菌素15m的方法，并给出了其作用机制的实验数据。数据表明，这种作用主要基于对甲硫氨酸生物合成途径的第一种酶——高丝氨酸-O-转琥珀酰酶的抑制，其方式类似于该氨基酸引起的别构抑制。
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