对苯并(a)芘致敏的豚鼠中对半抗原-皮肤蛋白结合物的迟发型超敏反应。
Delayed hypersensitivity to hapten-skin protein conjugates in guinea pigs sensitized to benzo(a)pyrene.
作者信息
Lewis F A, Heise E R, Tulis J J
出版信息
Int Arch Allergy Appl Immunol. 1978;57(6):535-41. doi: 10.1159/000232149.
Guinea pigs were sensitized to 3,4-benzo(a)pyrene, by epicutaneous application or by footbad injection in Freund's complete adjuvant. Conjugates of benzo(a)pyrene and guinea pig skin protein formed by ultraviolet radiation could elicit cutaneous delayed hypersensitivity and could inhibit the migration of macrophages obtained from guinea pigs sensitized to the carcinogen. Extracts of benzo(a)pyrene-treated guinea pig skin and conjugates formed in vitro with benzo(a)pyrene isocyanate were unable to consistently elicit delayed hypersensitivity reactions in vivo or in vitro. The results indicate a high degree of hapten-carrier specificity to contact sensitivity to benzo(a)pyrene.
通过在弗氏完全佐剂中经皮应用或足垫注射，使豚鼠对3,4-苯并(a)芘致敏。由紫外线辐射形成的苯并(a)芘与豚鼠皮肤蛋白的结合物可引发皮肤迟发型超敏反应，并可抑制从对致癌物致敏的豚鼠获得的巨噬细胞的迁移。经苯并(a)芘处理的豚鼠皮肤提取物以及在体外与异氰酸苯并(a)芘形成的结合物，在体内或体外均无法持续引发迟发型超敏反应。结果表明，对苯并(a)芘的接触敏感性存在高度的半抗原-载体特异性。
Zotero 插件