在蓖麻毒素内化过程中存在缺陷的中国仓鼠卵巢细胞突变体。
Chinese hamster ovary cell mutants defective in the internalization of ricin.
作者信息
Ray B, Wu H C
出版信息
Mol Cell Biol. 1982 May;2(5):535-44. doi: 10.1128/mcb.2.5.535-544.1982.
Abstract
Chinese hamster ovary mutants simultaneously resistant to ricin and Pseudomonas toxin have been isolated. Two mutant cell lines (4-10 and 11-2) were found to retain normal levels of binding of both ricin and Pseudomonas toxin. They were defective in the internalization of [125I]ricin into the mutant cells, as measured by both a biochemical assay for ricin internalization and electron microscopic autoradiographic studies. Although pretreatment of Chinese hamster ovary cells with a Na+/K+ ionophore, nigericin, resulted in an enhancement of the cytotoxicities of ricin and Pseudomonas toxin in the wild-type Chinese hamster ovary cells, preculture of the mutant cells did not alter the susceptibility of the mutant cells to either toxin. These results provide further evidence that there is a common step in the internalization process for ricin and Pseudomonas toxin.
摘要
已分离出对蓖麻毒素和铜绿假单胞菌毒素同时具有抗性的中国仓鼠卵巢突变体。发现两个突变细胞系（4 - 10和11 - 2）对蓖麻毒素和铜绿假单胞菌毒素的结合水平保持正常。通过蓖麻毒素内化的生化测定和电子显微镜放射自显影研究测量，它们在将[125I]蓖麻毒素内化到突变细胞中存在缺陷。尽管用Na⁺/K⁺离子载体尼日利亚菌素预处理中国仓鼠卵巢细胞会导致野生型中国仓鼠卵巢细胞中蓖麻毒素和铜绿假单胞菌毒素的细胞毒性增强，但突变细胞的预培养并未改变突变细胞对任何一种毒素的敏感性。这些结果进一步证明，蓖麻毒素和铜绿假单胞菌毒素的内化过程存在一个共同步骤。
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