尼日利亚菌素增强蓖麻毒素和铜绿假单胞菌毒素对中国仓鼠卵巢细胞的细胞毒性。
Enhancement of cytotoxicities of ricin and Pseudomonas toxin in Chinese hamster ovary cells by nigericin.
作者信息
Ray B, Wu H C
机构信息
Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20014.
出版信息
Mol Cell Biol. 1981 Jun;1(6):552-9. doi: 10.1128/mcb.1.6.552-559.1981.
Abstract
Nigericin and monensin, ionophores for Na+ and K+, have been found to enhance the cytotoxicities of abrin, ricin, and Pseudomonas aeruginosa exotoxin A in Chinese hamster ovary (CHO) cells. They do not affect the cytotoxicity of diphtheria toxin in the same cell line. Maximal sensitization of the CHO cells toward ricin and Pseudomonas toxin requires preculture of CHO cells in the presence of nigericin. Inhibition of protein synthesis in CHO cells by ricin or Pseudomonas toxin is also enhanced by preculture of CHO cells in the presence of nigericin. These results suggest a common step in the intoxication process of ricin and Pseudomonas toxin, the rate of which is facilitated by pretreatment with nigericin. This step is, however, not shared by the intoxication of CHO cells with diphtheria toxin.
摘要
尼日利亚菌素和莫能菌素是钠钾离子载体，已发现它们能增强相思子毒素、蓖麻毒素和铜绿假单胞菌外毒素A对中国仓鼠卵巢（CHO）细胞的细胞毒性。它们对同一细胞系中白喉毒素的细胞毒性没有影响。CHO细胞对蓖麻毒素和铜绿假单胞菌毒素的最大致敏作用需要在尼日利亚菌素存在的情况下对CHO细胞进行预培养。在尼日利亚菌素存在的情况下对CHO细胞进行预培养，也能增强蓖麻毒素或铜绿假单胞菌毒素对CHO细胞蛋白质合成的抑制作用。这些结果表明蓖麻毒素和铜绿假单胞菌毒素中毒过程有一个共同步骤，用尼日利亚菌素预处理可加快该步骤的速率。然而，白喉毒素使CHO细胞中毒的过程并不存在这一步骤。
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本文引用的文献
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Enhanced internalization of ricin in nigericin-pretreated Chinese hamster ovary cells.尼日利亚菌素预处理的中国仓鼠卵巢细胞中蓖麻毒素内化作用增强。
Mol Cell Biol. 1981 Jun;1(6):560-7. doi: 10.1128/mcb.1.6.560-567.1981.
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Inhibition of diphtheria toxin degradation and cytotoxic action by chloroquine.氯喹对白喉毒素降解及细胞毒性作用的抑制
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Ribosome inactivation by the toxic lectins abrin and ricin. Kinetics of the enzymic activity of the toxin A-chains.有毒凝集素相思子毒素和蓖麻毒素对核糖体的失活作用。毒素A链酶活性的动力学研究。
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Studies on the structure and properties of the lectins from Abrus precatorius and Ricinus communis.相思子和蓖麻凝集素的结构与性质研究。
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Plasma cell immunoglobulin secretion: arrest is accompanied by alterations of the golgi complex.浆细胞免疫球蛋白分泌：停滞伴随着高尔基体复合体的改变。
J Exp Med. 1977 Nov 1;146(5):1332-45. doi: 10.1084/jem.146.5.1332.
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The transport of lysosomal enzymes.溶酶体酶的运输
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Binding of ricin A chain to rat liver ribosomes: relationship to ribosome inactivation.蓖麻毒素A链与大鼠肝脏核糖体的结合：与核糖体失活的关系。
J Supramol Struct. 1978;9(2):253-68. doi: 10.1002/jss.400090210.
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Investigations into the relationship between structure and function of diphtheria toxin.对白喉毒素结构与功能关系的研究。
Proc Natl Acad Sci U S A. 1977 Feb;74(2):472-6. doi: 10.1073/pnas.74.2.472.
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