Ray B, Wu H C
Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20014.
Mol Cell Biol. 1981 Jun;1(6):552-9. doi: 10.1128/mcb.1.6.552-559.1981.
Nigericin and monensin, ionophores for Na+ and K+, have been found to enhance the cytotoxicities of abrin, ricin, and Pseudomonas aeruginosa exotoxin A in Chinese hamster ovary (CHO) cells. They do not affect the cytotoxicity of diphtheria toxin in the same cell line. Maximal sensitization of the CHO cells toward ricin and Pseudomonas toxin requires preculture of CHO cells in the presence of nigericin. Inhibition of protein synthesis in CHO cells by ricin or Pseudomonas toxin is also enhanced by preculture of CHO cells in the presence of nigericin. These results suggest a common step in the intoxication process of ricin and Pseudomonas toxin, the rate of which is facilitated by pretreatment with nigericin. This step is, however, not shared by the intoxication of CHO cells with diphtheria toxin.
尼日利亚菌素和莫能菌素是钠钾离子载体，已发现它们能增强相思子毒素、蓖麻毒素和铜绿假单胞菌外毒素A对中国仓鼠卵巢（CHO）细胞的细胞毒性。它们对同一细胞系中白喉毒素的细胞毒性没有影响。CHO细胞对蓖麻毒素和铜绿假单胞菌毒素的最大致敏作用需要在尼日利亚菌素存在的情况下对CHO细胞进行预培养。在尼日利亚菌素存在的情况下对CHO细胞进行预培养，也能增强蓖麻毒素或铜绿假单胞菌毒素对CHO细胞蛋白质合成的抑制作用。这些结果表明蓖麻毒素和铜绿假单胞菌毒素中毒过程有一个共同步骤，用尼日利亚菌素预处理可加快该步骤的速率。然而，白喉毒素使CHO细胞中毒的过程并不存在这一步骤。
