蓖麻毒素在中国仓鼠卵巢细胞中的内化作用。
Internalization of ricin in Chinese hamster ovary cells.
作者信息
Ray B, Wu H C
机构信息
Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20014.
出版信息
Mol Cell Biol. 1981 Jun;1(6):544-51. doi: 10.1128/mcb.1.6.544-551.1981.
Abstract
Internalization of ricin into Chinese hamster ovary cells has been investigated. Combined treatment with galactose and pronase at 0 degrees C resulted in a complete release of surface-bound [125I]ricin into the media. Galactose-pronase-resistant cell-bound [125I]ricin represents internalized ricin molecules inside the cells. The internalization process is time, temperature, and concentration dependent. The pH optimum of internalization of ricin is about pH 7. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis has revealed that intact ricin molecules are internalized. Neither reduction nor proteolytic processing of ricin is required for the entry of ricin into Chinese hamster ovary cells.
摘要
已对蓖麻毒素进入中国仓鼠卵巢细胞的内化过程进行了研究。在0摄氏度下用半乳糖和链霉蛋白酶联合处理，导致表面结合的[125I]蓖麻毒素完全释放到培养基中。半乳糖-链霉蛋白酶抗性的细胞结合[125I]蓖麻毒素代表细胞内内化的蓖麻毒素分子。内化过程与时间、温度和浓度有关。蓖麻毒素内化的最适pH约为pH 7。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示完整的蓖麻毒素分子被内化。蓖麻毒素进入中国仓鼠卵巢细胞不需要还原或蛋白水解处理。
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