来自黑猩猩肝脏的具有内源性DNA聚合酶活性的乙肝核心颗粒。

Hepatitis B core particles with endogenous DNA polymerase activity from chimpanzee liver.

作者信息

Onda H, Matsuda S, Oka Y

出版信息

Intervirology. 1978;10(4):254-64. doi: 10.1159/000148988.

Abstract

Hepatitis B core antigen (HBcAg) particles, approximately 27-28 nm in diameter and rho = 1.30-1.35 g/cm3, were purified from the liver of a chimpanzee experimentally infected with hepatitis B virus (HBV) while under cyclophosphamide treatment. The purified HBcAg particles incorporated radioactive deoxythymidine triphosphate. The product was precipitable by trichloroacetic acid and sensitive to DNase, but resistant to digestion by RNase. The reaction required four deoxyribonucleosise triphosphates- dATP, dCTP, dGTP and dTTP. Exogenous template did not enhance the reaction. From these findings, it was suggested that HBcAg particles purified from the HBV-infected chimpanzee liver contained DNA polymerase and endogenous DNA.

摘要

从一只在环磷酰胺治疗下实验感染乙型肝炎病毒（HBV）的黑猩猩肝脏中纯化出直径约27 - 28纳米、密度ρ = 1.30 - 1.35克/立方厘米的乙型肝炎核心抗原（HBcAg）颗粒。纯化的HBcAg颗粒掺入了放射性三磷酸脱氧胸苷。产物可被三氯乙酸沉淀，对DNA酶敏感，但对RNA酶消化有抗性。该反应需要四种脱氧核糖核苷三磷酸——dATP、dCTP、dGTP和dTTP。外源模板不能增强该反应。根据这些发现，提示从感染HBV的黑猩猩肝脏中纯化的HBcAg颗粒含有DNA聚合酶和内源性DNA。