Jacob J R, Eichberg J W, Lanford R E
Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78284.
Hepatology. 1989 Dec;10(6):921-7. doi: 10.1002/hep.1840100605.
Primary chimpanzee hepatocytes were maintained in vitro utilizing a serum-free medium. Hepatocyte functions were sustained throughout the culture period as demonstrated by the synthesis and secretion of liver-specific plasma proteins characteristic for differentiated hepatocytes. Hepatocyte cultures established from a chimpanzee chronically infected with human hepatitis B virus exhibited the synthesis and secretion of hepatitis B virus proteins into the medium. In addition, the de novo replication of hepatitis B virus was documented by the recovery of virus, exhibiting an endogenous DNA polymerase activity, from the tissue culture medium. Therefore, both the long-term maintenance of differentiated hepatocytes and the expression of hepatitis B virus from these primary cultures were sustained in the serum-free medium.
利用无血清培养基在体外培养原代黑猩猩肝细胞。在整个培养期间，肝细胞功能得以维持，这可通过分化肝细胞特有的肝脏特异性血浆蛋白的合成与分泌得到证明。从慢性感染人类乙型肝炎病毒的黑猩猩建立的肝细胞培养物表现出乙型肝炎病毒蛋白向培养基中的合成与分泌。此外，通过从组织培养基中回收具有内源性DNA聚合酶活性的病毒，证明了乙型肝炎病毒的从头复制。因此，在无血清培养基中，分化肝细胞的长期维持以及这些原代培养物中乙型肝炎病毒的表达均得以持续。
