Carp R I, Callahan S M
Intervirology. 1982;17(4):201-7. doi: 10.1159/000149289.
Mouse peritoneal macrophages were exposed to scrapie (ME7 strain) brain homogenate in vitro for 2 h at 37 degrees. The samples were assayed for infectivity by analysis of scrapie incubation periods and their values compared to those obtained after extended (1-28 days) in vitro incubation. The scrapie incubation periods for scrapie agent-macrophage mixtures which had undergone extended in vitro incubation were longer than for mixtures assayed after a 2-hour exposure to the scrapie agent. The difference in scrapie incubation periods was more dramatic when residual scrapie was eliminated by washing the cells after the 2-hour exposure to brain homogenate. The scrapie incubation period also increased following in vitro incubation of ME7 in the absence of cells; however, the changes were less than those observed for scrapie agent-macrophage mixtures. In a culture in which cells had been destroyed by UV irradiation after exposure to the scrapie agent, there was more scrapie infectivity than in a comparable culture of untreated, scrapie-exposed macrophages. These results show that scrapie infectivity decreases with extended incubation of scrapie-exposed macrophages, and the data suggest that macrophages can inactivate the scrapie agent in vitro.
将小鼠腹腔巨噬细胞在体外37℃下与羊瘙痒病（ME7毒株）脑匀浆接触2小时。通过分析羊瘙痒病潜伏期来检测样品的传染性，并将其值与延长（1 - 28天）体外培养后获得的值进行比较。经过延长体外培养的羊瘙痒病病原体 - 巨噬细胞混合物的羊瘙痒病潜伏期比暴露于羊瘙痒病病原体2小时后检测的混合物更长。当在接触脑匀浆2小时后通过洗涤细胞消除残留的羊瘙痒病病原体时，羊瘙痒病潜伏期的差异更为显著。在无细胞条件下对ME7进行体外培养后，羊瘙痒病潜伏期也会增加；然而，变化程度小于在羊瘙痒病病原体 - 巨噬细胞混合物中观察到的情况。在接触羊瘙痒病病原体后经紫外线照射破坏细胞的培养物中，羊瘙痒病传染性比未处理的、接触过羊瘙痒病病原体的巨噬细胞的对照培养物中更高。这些结果表明，暴露于羊瘙痒病病原体的巨噬细胞延长培养后，羊瘙痒病传染性降低，数据表明巨噬细胞可在体外使羊瘙痒病病原体失活。
