Schønheyder H, Andersen P, Stenderup A
Acta Pathol Microbiol Immunol Scand B. 1982 Aug;90(4):273-9. doi: 10.1111/j.1699-0463.1982.tb00118.x.
Aspergillus antibodies of the IgG, IgA and IgM class were determined by the indirect immunofluorescence (IIF) technique in sera from 11 patients with proven pulmonary aspergillosis (group I), 11 patients with suspected pulmonary aspergillosis (group II), 34 patients with chronic obstructive lung disease (group III), 29 patients with lung infiltrates of non-fungal aetiology (group IV), and 94 blood donors (group V). The antigen was cryostate sections of formaldehyde fixed mycelium. IgG antibody titres above 640 occurred in 73% in group I + II, 12% in group III, 0% in group IV, and 22% in group V. The difference between group I + II and group V was statistically significant (p less than 0.001). IgA antibody titres above 80 were only found in patients in group I + II, suggesting that demonstration of such antibodies may be of diagnostic significance. IgM antibodies in titres between 10 and 160 were found in 100-65% in group I to V. Precipitating antibodies to a culture filtrate antigen occurred in all subjects in group I, in 9 (82%) in group II, and in 1-7% of subjects in group III to V. However, precipitin titres above 2 occurred only in group I and II. No correlation between precipitin titres and IIF titres of IgG and IgA antibodies was obtained, suggesting that the two methods reveal antibodies of different specificities.
采用间接免疫荧光（IIF）技术测定了11例确诊肺曲霉病患者（I组）、11例疑似肺曲霉病患者（II组）、34例慢性阻塞性肺疾病患者（III组）、29例非真菌病因肺部浸润患者（IV组）以及94名献血者（V组）血清中IgG、IgA和IgM类曲霉抗体。抗原为甲醛固定菌丝体的冷冻切片。I组+II组中73%的患者IgG抗体滴度高于640，III组为12%，IV组为0%，V组为22%。I组+II组与V组之间的差异具有统计学意义（p<0.001）。仅在I组+II组患者中发现IgA抗体滴度高于80，提示此类抗体的检测可能具有诊断意义。I组至V组中，10至160滴度的IgM抗体在100%-65%的患者中存在。I组所有受试者、II组9例（82%）以及III组至V组1%-7%的受试者出现针对培养滤液抗原的沉淀抗体。然而，沉淀滴度高于2仅出现在I组和II组。未发现沉淀滴度与IgG和IgA抗体的IIF滴度之间存在相关性，提示这两种方法检测的抗体具有不同特异性。
