Williams P L, Moffat A C, King L J
J Chromatogr. 1978 Aug 11;155(2):273-83. doi: 10.1016/s0021-9673(00)87988-1.
A high-pressure liquid chromatography-radioimmunoassay (HPLC-RIA) method for the measurement of cannabinoid levels in plasma is described. The method is capable of quantifying 0.1 ng of a cannabinoid in 1 ml of plasma. The experimental procedure consists of an initial separation of cannabinoids in a plasma extract by HPLC followed by collection of the HPLC eluate and RIA. A chromatogram consisting of the cross-reacting cannabinoids in plasma may then be constructed. The plasma concentrations of cannabinoids with retention volumes equivalent to those of delta 9-tetrahydrocannabinol, cannabinol and mono-hydroxylated metabolites have been measured by this technique.
本文描述了一种用于测量血浆中大麻素水平的高压液相色谱-放射免疫分析法（HPLC-RIA）。该方法能够定量检测1毫升血浆中0.1纳克的大麻素。实验步骤包括先用HPLC对血浆提取物中的大麻素进行初步分离，然后收集HPLC洗脱液并进行放射免疫分析。接着可以构建一张由血浆中交叉反应性大麻素组成的色谱图。通过该技术已测量了保留体积与δ9-四氢大麻酚、大麻酚及单羟基化代谢物相当的大麻素的血浆浓度。
