使用强阴离子交换树脂改进核苷、核苷酸和氨酰基tRNA的分离。
Improved separation of nucleosides, nucleotides, and aminoacyl tRNA a strong anion-exchange resin.
作者信息
Kratovich M, Roe B A
出版信息
J Chromatogr. 1978 Aug 11;155(2):407-13. doi: 10.1016/s0021-9673(00)88002-4.
PMID:681493
Abstract
Nucleosides and nucleotides can be separated on a polystyrene-dinylbenzene anion-exchange resin, Chromex DA-X8-11 at 50 degree using isocratic elution with 1.0 M ammonium carbonate pH 8.8 or 0.05 M formic acid pH 2.4. The resin can also resolve aminoacyl tRNA on either the analytical or preparative scale.
摘要
核苷和核苷酸可在聚苯乙烯 - 二乙烯基苯阴离子交换树脂Chromex DA - X8 - 11上于50摄氏度进行分离，采用等度洗脱，流动相为pH 8.8的1.0 M碳酸铵或pH 2.4的0.05 M甲酸。该树脂也能在分析规模或制备规模上分离氨酰tRNA。
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