DeGrella R F, Simoni R D
J Biol Chem. 1982 Dec 10;257(23):14256-62.
We have modified a plasma membrane isolation procedure which utilizes DEAE-Sephadex beads (Gotlib, L. J., and Searls, D. B. (1980) Biochim. Biophys. Acta 602, 207-212) to rapidly measure intracellular transport of cholesterol from the site of synthesis in the endoplasmic reticulum to the plasma membrane. This transport process is rapid, with a half-time of about 10 min, has different kinetics from that of intracellular glycoprotein transport, and appears to be energy-dependent.
我们改进了一种利用二乙氨基乙基葡聚糖凝胶珠（戈特利布，L. J.，和西尔斯，D. B.（1980年）《生物化学与生物物理学学报》602卷，207 - 212页）的质膜分离程序，以快速测量胆固醇从内质网合成部位到质膜的细胞内转运。这个转运过程很快，半衰期约为10分钟，其动力学与细胞内糖蛋白转运不同，并且似乎是能量依赖的。
