Conley M E, Koopman W J
J Exp Med. 1982 Dec 1;156(6):1615-21. doi: 10.1084/jem.156.6.1615.
To investigate the in vitro regulation of IgA subclass synthesis, peripheral blood lymphocytes from healthy adults were cultured with the polyclonal B cell activator, pokeweed mitogen. Although 50% of the IgA plasma cells from a 7-d culture were positive for cytoplasmic IgA1 and 50% were positive for IgA2, less than 10% of the IgA released into the culture supernatant was IgA2. This discrepancy could not be explained by failure of the assay to detect in vitro synthesized IgA2, selective loss or destruction of IgA2 in culture media, delayed release of IgA2, or failure of IgA2 plasma cells to produce J chain. The results suggest that additional signals may be required for the differentiation of plasma cells into immunoglobulin-secreting cells.
为了研究体外对IgA亚类合成的调节，将来自健康成年人的外周血淋巴细胞与多克隆B细胞激活剂商陆丝裂原一起培养。尽管来自7天培养物的IgA浆细胞中有50%的细胞质IgA1呈阳性，50%的IgA2呈阳性，但释放到培养上清液中的IgA中只有不到10%是IgA2。这种差异无法用检测体外合成IgA2的试验失败、培养基中IgA2的选择性丢失或破坏、IgA2的延迟释放或IgA2浆细胞未能产生J链来解释。结果表明，浆细胞分化为免疫球蛋白分泌细胞可能需要额外的信号。
