Steeg P S, Moore R N, Johnson H M, Oppenheim J J
J Exp Med. 1982 Dec 1;156(6):1780-93. doi: 10.1084/jem.156.6.1780.
A culture supernatant of concanavalin A-activated spleen cells (Con A supernatant) induced murine macrophages to express Ia antigens in vitro. Biochemical characterization of the Con A supernatant indicated that the macrophage Ia antigen regulatory activity shares molecular weight, pI, and hydrophobic and affinity characteristics with immune interferon (IFN-gamma). Antiserum to mouse IFN-gamma neutralized both the macrophage Ia antigen regulatory and IFN-gamma bioactivities of the Con A supernatant. Furthermore, both partially purified murine IFN-gamma (10(7) U/mg protein sp act) and IFN-containing culture supernatants of the murine BFS T cell line-induced macrophage Ia antigen expression in vitro. Culture supernatants containing colony-stimulating factor, interleukin 1, interleukin 2, macrophage migration inhibitory factor, and a macrophage-activating activity that were distinct from IFN-gamma did not induce macrophage Ia antigen expression. Taken together, the data indicate that the in vitro expression of Ia antigens on macrophages is regulated by an activity that has the characteristics of interferon.
伴刀豆球蛋白A激活的脾细胞培养上清液（伴刀豆球蛋白A上清液）在体外诱导小鼠巨噬细胞表达Ia抗原。对伴刀豆球蛋白A上清液的生化特性分析表明，巨噬细胞Ia抗原调节活性在分子量、等电点、疏水性和亲和特性方面与免疫干扰素（IFN-γ）相同。抗小鼠IFN-γ血清可中和伴刀豆球蛋白A上清液的巨噬细胞Ia抗原调节活性和IFN-γ生物活性。此外，部分纯化的小鼠IFN-γ（比活性为10⁷U/mg蛋白）和小鼠BFS T细胞系含IFN的培养上清液均可在体外诱导巨噬细胞Ia抗原表达。含有集落刺激因子、白细胞介素1、白细胞介素2、巨噬细胞移动抑制因子以及一种与IFN-γ不同的巨噬细胞激活活性的培养上清液，均未诱导巨噬细胞Ia抗原表达。综上所述，数据表明巨噬细胞上Ia抗原的体外表达受一种具有干扰素特性的活性物质调节。
