一种不依赖白细胞介素2的小鼠T细胞系产生免疫干扰素。
Production of immune interferon by an interleukin 2-independent murine T cell line.
作者信息
Benjamin W R, Steeg P S, Farrar J J
出版信息
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5379-83. doi: 10.1073/pnas.79.17.5379.
An interleukin 2-independent murine T cell line (BFS) was isolated that produced immune interferon after stimulation with phorbol 12-myristate 13-acetate. The BFS cell line did not produce detectable levels of interleukin 1, interleukin 2, B cell growth factor, macrophage-granulocyte colony-stimulating factor, macrophage-activating factor, or T cell replacing factor. Maximal interferon was induced 48 hr after stimulation with phorbol myristate acetate at 10-100 ng/ml. Production of interferon by phorbol myristate acetate-stimulated BFS cell cultures was synergistically increased by the addition of EL4 thymoma cell culture supernatants. BFS-derived interferon activity was sensitive to pH 2 treatment and was neutralized with antiserum to immune interferon but was resistant to heating at 56 degrees C and to treatment with antiserum to type I interferon. In addition, the interferon activity was sensitive to trypsin but resistant to RNase. BFS-derived interferon had an apparent molecular weight of 48,000 and a pI of 5.5-6.0. Each of these properties is consistent with the conclusion that the BFS cell line produces immune interferon after stimulation with phorbol myristate acetate.
分离出一种白细胞介素2非依赖型小鼠T细胞系（BFS），该细胞系在用佛波酯12 -肉豆蔻酸酯13 -乙酸酯刺激后产生免疫干扰素。BFS细胞系不产生可检测水平的白细胞介素1、白细胞介素2、B细胞生长因子、巨噬细胞 - 粒细胞集落刺激因子、巨噬细胞激活因子或T细胞替代因子。在用10 - 100 ng/ml佛波酯肉豆蔻酸酯刺激48小时后诱导产生最大量的干扰素。通过添加EL4胸腺瘤细胞培养上清液，佛波酯肉豆蔻酸酯刺激的BFS细胞培养物中干扰素的产生协同增加。BFS衍生的干扰素活性对pH 2处理敏感，可被抗免疫干扰素血清中和，但对56℃加热和抗I型干扰素血清处理具有抗性。此外，干扰素活性对胰蛋白酶敏感，但对核糖核酸酶抗性。BFS衍生的干扰素表观分子量为48,000，pI为5.5 - 6.0。这些特性中的每一个都与BFS细胞系在用佛波酯肉豆蔻酸酯刺激后产生免疫干扰素的结论一致。
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