Leshem B, Naor D
J Immunol. 1978 Aug;121(2):401-8.
Heavy trinitrophenylated sheep red cells (TNP128SRC) and glutaraldehyde-treated SRC (G-SRC) could not induce cellular cytotoxicity against 51Cr-SRC. In contrast, the native antigen SRC could stimulate a cytolytic response against the radiolabeled homologous target cell. However, fixed SRC could stimulate a priming function that accelerated and augmented the secondary cytotoxic response to SRC. Such fixed antigens could stimulate a delayed-type hypersensitivity (DTHS) response also. Thus, the immunologic memory to the chemically modified antigen, as well as the DTHS response, are completely dissociated from the primary cytotoxic responses. The primary and the secondary cytotoxic responses that were developed in the spleens of the injected mice were mediated by antibody-dependent cellular cytotoxicity (ADCC), since the active supernatant that was released from the spleen cells could lyse the target cells in the presence of normal splenocytes. The active supernatant was identified as antibody. We suggest that B effector cells cytolyzed the antibody-coated target cells. Normal cells from nude mice could mediate the cytolytic process as efficiently as spleen cells from other strains of mouse. The results are discussed in terms of selective stimulation of T cell subpopulations.
重三硝基苯基化绵羊红细胞（TNP128SRC）和戊二醛处理的SRC（G-SRC）不能诱导针对51Cr-SRC的细胞毒性。相比之下，天然抗原SRC可刺激针对放射性标记的同源靶细胞的溶细胞反应。然而，固定的SRC可刺激引发功能，加速并增强对SRC的二次细胞毒性反应。这种固定抗原也可刺激迟发型超敏反应（DTHS）。因此，对化学修饰抗原的免疫记忆以及DTHS反应与初次细胞毒性反应完全分离。注射小鼠脾脏中产生的初次和二次细胞毒性反应由抗体依赖性细胞毒性（ADCC）介导，因为脾细胞释放的活性上清液在正常脾细胞存在下可裂解靶细胞。活性上清液被鉴定为抗体。我们认为B效应细胞溶解了抗体包被的靶细胞。裸鼠的正常细胞介导溶细胞过程的效率与其他品系小鼠的脾细胞相同。从T细胞亚群的选择性刺激方面对结果进行了讨论。
