近期流感抗原刺激后体外产生特异性流感抗体所致的细胞毒性。
Cell cytotoxicity due to specific influenza antibody production in vitro after recent influenza antigen stimulation.
作者信息
Greenberg S B, Six H R, Drake S, Couch R B
出版信息
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4622-6. doi: 10.1073/pnas.76.9.4622.
Abstract
Peripheral blood leukocytes, obtained from volunteers after vaccination or natural illness with influenza, were assayed for cytotoxicity against influenza virus-infected cells. Approximately 7 days after vaccination or the onset of respiratory illness, peak cytotoxicity was demonstrated in a chromium-release assay. Secretion of specific antibody against hemagglutinin from the leukocytes during in vitro incubation was demonstrated in quantities that would mediate the cell cytotoxicity observed. Antibody secretion was inhibited by exposure to cycloheximide but not by exposure to trypsin. The secretion of antibody against hemagglutinin from peripheral blood leukocytes occurred only at the time of maximal cytotoxicity. We thus demonstrate secretion of specific antibody in vitro after recent viral antigen stimulation. Moreover, this antibody is capable of conveying cytotoxic capacity to peripheral blood leukocytes that may be important in the recovery process from acute viral infection.
摘要
从接种流感疫苗或自然感染流感的志愿者身上获取外周血白细胞，检测其对流感病毒感染细胞的细胞毒性。在接种疫苗或出现呼吸道疾病约7天后，铬释放试验显示出峰值细胞毒性。体外培养期间，白细胞分泌的针对血凝素的特异性抗体量足以介导所观察到的细胞毒性。抗体分泌受环己酰亚胺抑制，但不受胰蛋白酶抑制。外周血白细胞中针对血凝素的抗体分泌仅在最大细胞毒性时出现。因此，我们证明了近期病毒抗原刺激后体外特异性抗体的分泌。此外，这种抗体能够赋予外周血白细胞细胞毒性能力，这在急性病毒感染的恢复过程中可能很重要。
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