脂多糖刺激对细胞表面免疫球蛋白M信使核糖核酸转录和翻译的影响。
Effect of lipopolysaccharide stimulation on the transcription and translation of messenger RNA for cell surface immunoglobulin M.
作者信息
Yuan D, Tucker P W
出版信息
J Exp Med. 1982 Oct 1;156(4):962-74. doi: 10.1084/jem.156.4.962.
Abstract
Analysis of mu-specific mRNA in the B cell tumor line, BCL1, shows that the cells contain predominantly mRNA for mu chain of membrane-bound immunoglobulin M (IgM) (2.7 kb, mu m mRNA). Stimulation of the cells to Ig secretion by lipopolysaccharide (LPS) results in a 6-12 fold increase in amount of mRNA for the mu chain of secreted IgM (2.4 kb mu s mRNA). The increase in mu s mRNA is accompanied by a 3-4-fold increase in mu m mRNA. The rate of mu chain synthesis of membrane IgM in LPS-stimulated cells is, however, reduced by at least twofold, suggesting that both transcriptional and translational regulatory events are involved in the induction of B lymphocytes to secretion.
摘要
对B细胞肿瘤系BCL1中μ特异性mRNA的分析表明，这些细胞主要含有膜结合免疫球蛋白M（IgM）μ链的mRNA（2.7 kb，μm mRNA）。用脂多糖（LPS）刺激这些细胞使其分泌Ig，会导致分泌型IgM μ链的mRNA量增加6至12倍（2.4 kb μs mRNA）。μs mRNA的增加伴随着μm mRNA增加3至4倍。然而，LPS刺激的细胞中膜IgM的μ链合成速率至少降低了两倍，这表明转录和翻译调控事件都参与了B淋巴细胞向分泌状态的诱导过程。
相似文献
1
Effect of lipopolysaccharide stimulation on the transcription and translation of messenger RNA for cell surface immunoglobulin M.脂多糖刺激对细胞表面免疫球蛋白M信使核糖核酸转录和翻译的影响。
J Exp Med. 1982 Oct 1;156(4):962-74. doi: 10.1084/jem.156.4.962.
2
IgM RNA switch from membrane to secretory form is prevented by adding antireceptor antibody to bacterial lipopolysaccharide-stimulated murine primary B-cell cultures.通过向细菌脂多糖刺激的小鼠原代B细胞培养物中添加抗受体抗体，可阻止IgM RNA从膜形式转变为分泌形式。
Proc Natl Acad Sci U S A. 1985 Nov;82(21):7384-8. doi: 10.1073/pnas.82.21.7384.
3
4
Regulation of microM vs microS mRNA expression in an inducible B cell line.
Mol Immunol. 1989 Nov;26(11):1059-67. doi: 10.1016/0161-5890(89)90070-9.
5
Transcriptional control of mu- and kappa-gene expression in resting and bacterial lipopolysaccharide-activated normal B cells.静息和细菌脂多糖激活的正常B细胞中μ和κ基因表达的转录调控
Immunobiology. 1987 Mar;174(2):162-76. doi: 10.1016/s0171-2985(87)80036-0.
6
Regulation of membrane IgM expression in secretory B cells: translational and post-translational events.分泌型B细胞中膜IgM表达的调控：翻译及翻译后事件
EMBO J. 1987 Dec 20;6(13):3969-77. doi: 10.1002/j.1460-2075.1987.tb02739.x.
7
Regulation of IgM and IgD expression in human B-lineage cells.人类B淋巴细胞系细胞中IgM和IgD表达的调控
J Immunol. 1991 May 15;146(10):3314-21.
8
9
Role of an RNA cleavage/poly(A) addition site in the production of membrane-bound and secreted IgM mRNA.RNA 切割/聚腺苷酸化位点在膜结合型和分泌型 IgM mRNA 产生中的作用。
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8658-62. doi: 10.1073/pnas.82.24.8658.
10
Regulation of immunoglobulin gene expression in normal lymphocytes. II. Mechanisms of down-regulation of immunoglobulin secretion after engagement of the B cell antigen receptor.
Eur J Immunol. 1992 Feb;22(2):525-9. doi: 10.1002/eji.1830220235.
引用本文的文献
1
Comprehensive analysis of immunoglobulin expression in the mouse brain from embryonic to adult stages.对小鼠从胚胎期到成年期大脑中免疫球蛋白表达的综合分析。
J Neuroinflammation. 2025 Jun 9;22(1):153. doi: 10.1186/s12974-025-03457-9.
2
Differential regulation of histone acetylation and generation of mutations in switch regions is associated with Ig class switching.组蛋白乙酰化的差异调节以及转换区突变的产生与免疫球蛋白类别转换相关。
Proc Natl Acad Sci U S A. 2004 Oct 26;101(43):15428-33. doi: 10.1073/pnas.0406827101. Epub 2004 Oct 14.
3
Repression of c-myc is necessary but not sufficient for terminal differentiation of B lymphocytes in vitro.c-myc的抑制对于体外B淋巴细胞的终末分化是必要的，但并不充分。
Mol Cell Biol. 2000 Dec;20(23):8684-95. doi: 10.1128/MCB.20.23.8684-8695.2000.
4
In vitro enhancement of immunoglobulin gene expression in chronic lymphocytic leukemia.慢性淋巴细胞白血病中免疫球蛋白基因表达的体外增强
J Clin Invest. 1984 Feb;73(2):587-92. doi: 10.1172/JCI111247.
5
Transcriptional regulation of the mu-delta heavy chain locus in normal murine B lymphocytes.正常小鼠B淋巴细胞中μ-δ重链基因座的转录调控。
J Exp Med. 1984 Aug 1;160(2):564-83. doi: 10.1084/jem.160.2.564.
6
Low-grade lymphomas. Expression of developmentally regulated B-cell antigens.低度恶性淋巴瘤。发育调控性B细胞抗原的表达。
Am J Pathol. 1984 Apr;115(1):117-24.
7
Inducible transcription of the unrearranged kappa constant region locus is a common feature of pre-B cells and does not require DNA or protein synthesis.未重排的κ恒定区基因座的可诱导转录是前B细胞的一个共同特征，且不需要DNA或蛋白质合成。
Proc Natl Acad Sci U S A. 1985 Aug;82(16):5305-9. doi: 10.1073/pnas.82.16.5305.
8
Pretranslational regulation of the synthesis of the third component of complement in human mononuclear phagocytes by the lipid A portion of lipopolysaccharide.脂多糖的脂质A部分对人单核吞噬细胞中补体第三成分合成的转录前调控。
J Clin Invest. 1985 Sep;76(3):985-90. doi: 10.1172/JCI112099.
9
Regulation of membrane IgM expression in secretory B cells: translational and post-translational events.分泌型B细胞中膜IgM表达的调控：翻译及翻译后事件
EMBO J. 1987 Dec 20;6(13):3969-77. doi: 10.1002/j.1460-2075.1987.tb02739.x.
10
Mechanism of suppression of lipopolysaccharide-driven B cell differentiation by anti-mu antibodies. Evidence for a trans-acting repressor of transcription.抗μ抗体抑制脂多糖驱动的B细胞分化的机制。转录反式作用阻遏物的证据。
J Exp Med. 1987 Oct 1;166(4):864-73. doi: 10.1084/jem.166.4.864.
本文引用的文献
1
Preferential stimulation of ribosomal protein synthesis by insulin and in the absence of ribosomal and messenger ribonucleic acid formation.胰岛素对核糖体蛋白合成的优先刺激作用，且此过程不伴有核糖体和信使核糖核酸的形成。
Biochemistry. 1980 Apr 15;19(8):1662-9. doi: 10.1021/bi00549a022.
2
Post-transciptional control of coordinated ribosomal protein synthesis in Escherichia coli.大肠杆菌中核糖体蛋白合成协同的转录后调控
Nature. 1980 Feb 7;283(5747):599-600. doi: 10.1038/283599a0.
3
Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines.艾贝尔逊鼠白血病病毒转化的淋巴样细胞系中的多种免疫球蛋白重链基因转录物
Mol Cell Biol. 1982 Apr;2(4):386-400. doi: 10.1128/mcb.2.4.386-400.1982.
4
Organization and expression of immunoglobulin genes in fetal liver hybridomas.胎儿肝脏杂交瘤中免疫球蛋白基因的组织与表达
Proc Natl Acad Sci U S A. 1981 Jan;78(1):247-51. doi: 10.1073/pnas.78.1.247.
5
6
In vitro translation of Drosophila heat-shock and non--heat-shock mRNAs in heterologous and homologous cell-free systems.果蝇热休克和非热休克mRNA在异源和同源无细胞系统中的体外翻译。
Cell. 1981 Feb;23(2):595-603. doi: 10.1016/0092-8674(81)90155-0.
7
B lymphocyte differentiation and the control of IgM mu chain expression.B淋巴细胞分化与IgMμ链表达的调控
Cell. 1981 Feb;23(2):379-89. doi: 10.1016/0092-8674(81)90133-1.
8
Biosynthesis of two forms of IgM heavy chains by normal mouse B lymphocytes. Membrane and secretory IgM.正常小鼠B淋巴细胞合成两种形式的IgM重链。膜型和分泌型IgM。
J Biol Chem. 1980 Dec 25;255(24):11822-7.
9
5' flanking region of immunoglobulin heavy chain constant region genes displays length heterogeneity in germlines of inbred mouse strains.免疫球蛋白重链恒定区基因的5'侧翼区域在近交系小鼠品系的种系中表现出长度多态性。
Cell. 1980 Nov;22(1 Pt 1):187-96. doi: 10.1016/0092-8674(80)90167-1.
10
The immunoglobulin mu chains of membrane-bound and secreted IgM molecules differ in their C-terminal segments.膜结合型和分泌型IgM分子的免疫球蛋白μ链在其C末端片段上有所不同。
Cell. 1980 Sep;21(2):393-406. doi: 10.1016/0092-8674(80)90476-6.
Zotero 插件