Boyd A W, Goding J W, Schrader J W
J Immunol. 1981 Jun;126(6):2461-5.
The B cell lymphoma WEHI 231 has been previously characterized as resembling a resting B cell, bearing large amounts of IgM on the cell surface, but not secreting immunoglobulin. When WEHI 231 cells were cultured together with low concentrations of lipopolysaccharide (LPS) (0.01 to 3 micrograms/ml), increased levels of immunoglobulin were detected in culture supernatants. Analysis by immunoprecipitation and gel electrophoresis demonstrated that this was secreted (19S) IgM. It was noted that small amounts of secretory IgM were produced even in control cultures not deliberately exposed to LPS. Analysis of the cell lysates showed that LPS treatment resulted in a reduction of synthesis of the surface form of IgM. In contrast, the cytoplasmic pool of IgM precursors was considerably expanded by LPS treatment, reflecting the overall increase in synthesis of IgM in LPS-treated cells. These changes in IgM metabolism appear to parallel closely those occurring in normal B cells after mitogen or antigen challenge. This cloned tumor line promises to be a valuable system in which to investigate some of the molecular events in B cell differentiation.
B细胞淋巴瘤WEHI 231先前被鉴定为类似于静止B细胞，在细胞表面带有大量IgM，但不分泌免疫球蛋白。当WEHI 231细胞与低浓度脂多糖（LPS）（0.01至3微克/毫升）一起培养时，在培养上清液中检测到免疫球蛋白水平升高。通过免疫沉淀和凝胶电泳分析表明，这是分泌型（19S）IgM。值得注意的是，即使在未特意暴露于LPS的对照培养物中也产生了少量分泌型IgM。对细胞裂解物的分析表明，LPS处理导致IgM表面形式的合成减少。相反，LPS处理使IgM前体的细胞质池显著扩大，反映了LPS处理细胞中IgM合成的总体增加。IgM代谢的这些变化似乎与有丝分裂原或抗原刺激后正常B细胞中发生的变化密切平行。这个克隆的肿瘤细胞系有望成为研究B细胞分化中一些分子事件的有价值的系统。
