Makinde A A, Wilkie B N
Can J Comp Med. 1979 Jan;43(1):68-77.
Rabbits were infected with Dermatophilus congolensis and tested for humoral immune response by indirect haemagglutination and for cell-mediated immune response to crude antigens of D. congolensis. Lymphocyte transformation and macrophage migration inhibition assays were used as in vitro correlates of cell-mediated immune response while cutaneous delayed hypersensitivity was used in vivo. Endo-antigen and whole cell antigen were found to significantly induce cell-mediated immune response. In contrast, humoral responses were found to be more significantly induced by exo-antigen. A biphasic immune response was revealed by the lymphocyte transformation test.
将兔感染刚果嗜皮菌，并通过间接血凝试验检测其体液免疫反应，以及对刚果嗜皮菌粗抗原的细胞介导免疫反应。淋巴细胞转化试验和巨噬细胞移动抑制试验被用作细胞介导免疫反应的体外相关指标，而皮肤迟发型超敏反应则用于体内检测。发现内抗原和全细胞抗原能显著诱导细胞介导免疫反应。相比之下，外抗原能更显著地诱导体液反应。淋巴细胞转化试验显示出双相免疫反应。
