The determination of total bilirubin with the Du Pont ACA with respect to neonatal sera.

The interference of hemoglobin in the determination of total bilirubin with the Du Pont ACA was studied. The method seemed of limited value in our opinion especially with respect to neonatal sera. By changing the wave-length setting a rather broad plateau was found when the hemoglobin concentration was plotted against the actual bilirubin concentration. On the basis of these findings a new calibration graph was constructed and programmed into the ACA. The new method correlates well with two other micro bilirubin techniques.