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蟾蜍膀胱短路电流和活性磷酸盐转运的pH依赖性

pH dependence of short-circuit current and active phosphate transport by toad bladder.

作者信息

Mendoza S A, Hall J A, Sellers B B

出版信息

Miner Electrolyte Metab. 1982 Apr;7(4):219-24.

PMID:6820474
Abstract

The effect of changing the pH of the mucosal and serosal bathing solutions on active Na transport (estimated by the short-circuit current) and active inorganic phosphate (Pi) transport was studied in the toad bladder. The short-circuit current was unaffected by lowering the pH from 7.6 to 7.1 but was inhibited (29-45%) at pH 6.6, 8.1, 8.6, 9.1, or 9.6. The Pi transport was not changed when the pH was increased from 7.6 to 8.1, but was totally inhibited at pH 6.6, 7.1, 8.6, 9.1 or 9.6. Thus, active transport of Pi occurs only when most of the Pi is HPO4(-2), but the effect of varying pH on active Pi transport in the toad bladder is more complicated than a simple titration of Pi.

摘要

在蟾蜍膀胱中研究了改变黏膜和浆膜浴液的pH值对主动钠转运(通过短路电流估算)和主动无机磷酸盐(Pi)转运的影响。将pH值从7.6降至7.1时,短路电流不受影响,但在pH值为6.6、8.1、8.6、9.1或9.6时受到抑制(29 - 45%)。当pH值从7.6升至8.1时,Pi转运没有变化,但在pH值为6.6、7.1、8.6、9.1或9.6时完全受到抑制。因此,只有当大部分Pi为HPO4(-2)时才会发生Pi的主动转运,但蟾蜍膀胱中pH值变化对主动Pi转运的影响比简单的Pi滴定更为复杂。

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