Yasueda H, Yui Y, Shimizu T, Shida T
J Allergy Clin Immunol. 1983 Jan;71(1 Pt 1):77-86. doi: 10.1016/0091-6749(83)90550-x.
A purified allergen, antigen SBP, was obtained from the pollen of the Japanese cedar (Cryptomeria japonica, Sugi in Japanese) by ammonium sulfate precipitation, ion-exchange chromatography on diethylaminoethyl and carboxymethyl cellulose, and gel chromatography on Sephadex G-150. Antigen SBP was a heat-sensitive basic glycoprotein of approximately 40,000 molecular weight. By preparative isoelectric focusing and gel chromatography on Sephadex G-100, antigen SBP could be further separated into four subfractions, differing in both isoelectric point and molecular weight. By immunodiffusion analysis, direct skin testing, and radioallergosorbent test inhibition, it was shown that antigen SBP was the major allergen of Japanese cedar pollen, and the four subfractions were seen to be antigenically and allergenically identical.
通过硫酸铵沉淀、在二乙氨基乙基纤维素和羧甲基纤维素上进行离子交换色谱以及在葡聚糖凝胶G - 150上进行凝胶色谱，从日本柳杉（Cryptomeria japonica，日语为Sugi）花粉中获得了一种纯化的变应原，即抗原SBP。抗原SBP是一种分子量约为40,000的热敏感碱性糖蛋白。通过制备性等电聚焦和在葡聚糖凝胶G - 100上进行凝胶色谱，抗原SBP可进一步分离为四个亚组分，其等电点和分子量均不同。通过免疫扩散分析、直接皮肤试验和放射变应原吸附试验抑制，表明抗原SBP是日本柳杉花粉的主要变应原，并且这四个亚组分在抗原性和变应原性上是相同的。
