Thompson C B, Jakubowski J A, Quinn P G, Deykin D, Valeri C R
J Lab Clin Med. 1983 Feb;101(2):205-13.
The relationship between MPV and platelet function was studied in human platelet subpopulations separated on the basis of size and counterflow centrifugation. The original platelet population and five size-dependent platelet fractions were suspended in buffer or autologous PPP at a platelet count of 2 x 10(8)/ml. Collagen (10 micrograms/ml)-induced aggregation showed a significant negative correlation between MPV and onset of aggregation and positive correlations between the MPV and the rate of extent of aggregation. Thrombin stimulation (1 U/ml) demonstrated similar relationships between MPV and the rate of extent of aggregation. In contrast, ristocetin-induced platelet agglutination occurred at a similar rate and extent in all fractions. To quantitate further the differential response of the platelets, we measured the content and release of ATP and beta-TG. There was a significant correlation between MPV and both ATP and beta-TG content, and a progressive increase in the absolute release of ATP and beta-TG was associated with the increase in MPV through the fractions after stimulation. However, the percent release of total ATP and beta-TG was similar in all fractions. Our data indicate that the intrinsic function of platelets of different sizes is similar but that the absolute ability of platelets to affect their environment, as measured by aggregation and total release of granular content, correlates with their size.
基于大小和逆流离心法分离出人类血小板亚群，研究了平均血小板体积（MPV）与血小板功能之间的关系。将原始血小板群体和五个与大小相关的血小板组分悬浮于缓冲液或自体血小板血浆（PPP）中，血小板计数为2×10⁸/ml。胶原蛋白（10微克/毫升）诱导的聚集显示，MPV与聚集起始之间存在显著负相关，MPV与聚集速率和程度之间存在正相关。凝血酶刺激（1单位/毫升）显示MPV与聚集速率和程度之间存在类似关系。相比之下，瑞斯托霉素诱导的血小板凝集在所有组分中的发生速率和程度相似。为了进一步量化血小板的差异反应，我们测量了三磷酸腺苷（ATP）和β-血小板球蛋白（β-TG）的含量及释放情况。MPV与ATP和β-TG含量均存在显著相关性，刺激后各组分中，随着MPV的增加，ATP和β-TG的绝对释放量逐渐增加。然而，所有组分中ATP和β-TG的总释放百分比相似。我们的数据表明，不同大小血小板的内在功能相似，但通过聚集和颗粒内容物的总释放来衡量，血小板影响其环境的绝对能力与其大小相关。
