Sims N R, Bowen D M, Allen S J, Smith C C, Neary D, Thomas D J, Davison A N
J Neurochem. 1983 Feb;40(2):503-9. doi: 10.1111/j.1471-4159.1983.tb11311.x.
Indices of presynaptic cholinergic nerve endings were assayed in neocortical biopsy samples from patients with presenile dementia. For those patients in whom Alzheimer's disease was histologically confirmed, [14C]acetylcholine synthesis, choline acetyltransferase activity and choline uptake were all found to be markedly reduced (at least 40%) below mean control values. The changes occurred in samples from both the frontal and temporal lobes and for [14C]acetylcholine synthesis the decrease was similar under conditions of high and low neuronal activity (as assessed by incubations in 31 mM and 5 mM K+ respectively). Samples from other demented patients, in whom the histological features of Alzheimer's disease were not detected, produced values for all three biochemical parameters which were similar to controls. For the total group of patients with presenile dementia there were correlations between values for the three markers of presynaptic cholinergic nerve endings suggestive of a loss of functional activity at these sites in Alzheimer's disease.
对早老性痴呆患者的新皮质活检样本中突触前胆碱能神经末梢指标进行了测定。对于那些经组织学确诊为阿尔茨海默病的患者，发现[14C]乙酰胆碱合成、胆碱乙酰转移酶活性和胆碱摄取均显著低于平均对照值（至少降低40%）。这些变化发生在额叶和颞叶的样本中，对于[14C]乙酰胆碱合成，在高神经元活性和低神经元活性条件下（分别通过在31 mM和5 mM K+中孵育评估）降低程度相似。在其他未检测到阿尔茨海默病组织学特征的痴呆患者样本中，所有三个生化参数的值与对照组相似。对于早老性痴呆患者的总体而言，突触前胆碱能神经末梢的三个标志物的值之间存在相关性，提示在阿尔茨海默病中这些部位存在功能活性丧失。
