Berger T, Marrs R P, Saito H, Kletzky O A
Am J Obstet Gynecol. 1983 Feb 15;145(4):397-401. doi: 10.1016/0002-9378(83)90306-x.
The effects of in vivo and in vitro aging of hamster ova, protein supplementation of culture media, sperm concentration, and sperm motility on penetration of zona-free hamster ova by human sperm were investigated. Penetrability of ova was significantly lowered by 2 to 4 additional hours of in vivo aging or by an additional 3 hours of in vitro aging. A comparison was made of the effects of the addition to the media of human preovulatory serum and human serum albumin, and the penetrating ability of human sperm was increased with the addition of 10% human preovulatory serum. Sperm motility was also better maintained in the presence of 10% serum. Maximum penetration occurred after 3 hours of sperm-egg interaction following a 3-hour preincubation period with a sperm concentration of 5 x 10(6) motile sperm per milliliter. When motile sperm concentration was maintained at 1 X 10(7) motile sperm per milliliter, there was no correlation between penetrating ability and percentage of motility. These factors should be controlled to allow reproducible results with the hamster test.
研究了仓鼠卵的体内和体外老化、培养基的蛋白质补充、精子浓度以及精子活力对人精子穿透去透明带仓鼠卵的影响。体内额外老化2至4小时或体外额外老化3小时会使卵的可穿透性显著降低。比较了向培养基中添加人排卵前血清和人血清白蛋白的效果，添加10%的人排卵前血清可提高人精子的穿透能力。在10%血清存在的情况下，精子活力也能得到更好的维持。在每毫升5×10⁶个活动精子的精子浓度下预孵育3小时后，精子与卵子相互作用3小时后出现最大穿透率。当活动精子浓度维持在每毫升1×10⁷个活动精子时，穿透能力与活力百分比之间没有相关性。应控制这些因素，以便在仓鼠试验中获得可重复的结果。
