Binor Z, Rao R, van der Ven H, Scommegna A
Fertil Steril. 1982 Aug;38(2):222-6. doi: 10.1016/s0015-0282(16)46463-0.
The motility and the capability to penetrate zona-free hamster eggs of Y-enriched or washed sperm were evaluated after protracted in vitro incubation with and without the addition of preheated human serum. The addition of 50% preheated serum decreased the motility loss over time for both the washed and Y-enriched sperm. Such motility loss was decreased by 25% and 27% at 20 hours, by 31% and 39% at 30 hours, and by 30% and 40% at 40 hours of incubation for the washed and Y-enriched sperm, respectively. The washed and Y-enriched sperm suspensions with and without addition of preheated human serum achieved 100% penetration rate after 2 hours of preincubation. However, when scored by the sperm per egg ratio, washed sperm achieved 1.2 +/- 0.2 (mean +/- standard error [SE]) sperm per egg, while the Y-enriched sperm achieved 3.0 +/- 0.4 sperm per egg. After 24 hours of incubation, penetration by washed sperm decreased to a mean of 62.8%. The Y-enriched sperm penetrated a mean of 18% of the ova. Addition of preheated serum increased the egg penetrating capacity of washed sperm at 24 hours but failed to improve the Y-enriched spermatozoa. This study suggests that for optimum conception rates, precise ovulation timing is crucial when Y-enriched fractions are used for insemination.
在添加或不添加预热人血清的情况下，对富含Y染色体或经洗涤的精子进行长时间体外孵育后，评估其活力以及穿透去透明带仓鼠卵的能力。添加50%的预热血清可减少经洗涤精子和富含Y染色体精子随时间的活力损失。对于经洗涤精子和富含Y染色体精子，在孵育20小时时，这种活力损失分别减少了25%和27%；在30小时时，分别减少了31%和39%；在40小时时，分别减少了30%和40%。添加和不添加预热人血清的经洗涤精子和富含Y染色体精子悬浮液在预孵育2小时后均达到了100%的穿透率。然而，以每个卵子的精子数来计分，经洗涤精子每个卵子的精子数为1.2±0.2（平均值±标准误[SE]），而富含Y染色体精子每个卵子的精子数为3.0±0.4。孵育24小时后，经洗涤精子的穿透率降至平均62.8%。富含Y染色体精子的平均穿透率为18%。添加预热血清可提高经洗涤精子在24小时时的卵子穿透能力，但未能改善富含Y染色体精子的情况。本研究表明，为获得最佳受孕率，当使用富含Y染色体的部分进行授精时，精确的排卵时间至关重要。
