Veselá D, Veselý D, Jelínek R
Appl Environ Microbiol. 1983 Jan;45(1):91-3. doi: 10.1128/aem.45.1.91-93.1983.
The embryotoxic potential of ochratoxin A and citrinin was studied after administering, either subgerminally or intraamniotically, single mounting doses of the mycotoxins to chicken embryos on days 2, 3, and 4. The beginning of the embryotoxicity dose range was found to be between 0.01 to 0.05 microgram for ochratoxin A and 1 to 10 micrograms for citrinin. The maximum response to both mycotoxins occurred after administration on day 3. In addition to significant growth retardation of fetuses, exencephaly, microphthalmia, cleft beak, reduction deformities of the limbs, and abdominal wall and ventricular septal defects were encountered on day 8 of incubation. When 4 micrograms of citrinin was constantly added to ochratoxin A administered in the dose range of 0.03 to 0.5 microgram, a strictly additive effect was seen. It may be supposed that citrinin produced together with ochratoxin A in some strains of Penicillium viridicatum Westling does not potentiate the clear-cut embryotoxic action of the latter mycotoxin.
在第2、3和4天，通过胚下或羊膜内给鸡胚单次递增剂量的霉菌毒素，研究了赭曲霉毒素A和桔霉素的胚胎毒性潜力。发现赭曲霉毒素A的胚胎毒性剂量范围起始于0.01至0.05微克，桔霉素为1至10微克。两种霉菌毒素的最大反应均出现在第3天给药后。除了胎儿明显生长迟缓外，在孵化第8天还出现了无脑畸形、小眼畸形、喙裂、肢体减少畸形以及腹壁和室间隔缺损。当在0.03至0.5微克剂量范围内给赭曲霉毒素A持续添加4微克桔霉素时，观察到严格的相加效应。可以推测，在某些绿青霉菌株中与赭曲霉毒素A一起产生的桔霉素不会增强后一种霉菌毒素明显的胚胎毒性作用。
