Stephens-Jarnagin A, Sietsema W K, Miller D A, DeLuca H F
Arch Biochem Biophys. 1983 Feb 1;220(2):502-8. doi: 10.1016/0003-9861(83)90441-1.
Vitamin A-deficient rats were given a single intrajugular injection of 1 mg all-trans-[11-3H]retinoic acid and 3 h later the rats were killed. The small intestines were extracted and chromatographed by high-performance liquid chromatography to yield distinct metabolites. These were quantitated using the assumption that the specific activity of the metabolite is equal to that of the parent [3H]retinoic acid. The biological activity of all discernible metabolites was determined in the vitamin A-deficient female rat by vaginal smear assay. Retinoic acid and retinoyl-beta-glucuronide from the preparation had equal activity while no activity was found for any of the other metabolite fractions. Thus, no evidence for an unknown metabolite having potent epithelial differentiating activity could be found in this target tissue of vitamin A action.
给维生素A缺乏的大鼠颈内静脉单次注射1毫克全反式-[11-³H]视黄酸，3小时后处死大鼠。取出小肠，通过高效液相色谱法进行层析，得到不同的代谢产物。使用代谢产物的比活性等于母体[³H]视黄酸的比活性这一假设对这些代谢产物进行定量。通过阴道涂片试验在维生素A缺乏的雌性大鼠中测定所有可识别代谢产物的生物活性。制剂中的视黄酸和视黄酰-β-葡萄糖醛酸具有同等活性，而其他任何代谢产物组分均未发现活性。因此，在这个维生素A作用的靶组织中未发现有未知代谢产物具有强大上皮分化活性的证据。
